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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLDVLKFNFK FSQVVSVFVS NSSNMQKEIA GDAPSAPTTR VRHRKRNSDV GAGAGKPNGN

70 80 90 100 110 120
HLLVNDSKKY KSFLIRAYST FWMIGGFALI VYLGHLYITA MVVVIQIFMA RELFNLLRKT

130 140 150 160 170 180
HEDKQLPGFR LLNWHFFFTA MLFVYGRILS QRLVNTVTPD KVLYRLVTSL IKYHMAICYS

190 200 210 220 230 240
LYISGFVWFI LTLKKKMYKY QFSQYAWTHM ILIVVFTQSS FTVANIFEGI FWFLLPASLI

250 260 270 280 290 300
VINDIFAYIC GFFFGRTPLI KLSPKKTWEG FIGASITTVI SAFLLANIMG RFLWLTCPRE

310 320 330 340 350 360
DLSTGWLLCD PGPLFKQETH ALPGWISDWL PWKEISILPV QWHALCLGLF ASIIAPFGGF

370 380 390 400 410 420
FASGFKRAFK IKDFGDSIPG HGGITDRMDC QMVMAVFAYI YHQSFVVPEV LSVDKLLDQI

430 440
ITSLTLEEQQ ALLVKLGQML QEKVIGS


References and documentation are available.

Number of amino acids: 447 Molecular weight: 50966.20 Theoretical pI: 9.47
Amino acid composition: 
Ala (A) 27 6.0% Arg (R) 16 3.6% Asn (N) 13 2.9% Asp (D) 16 3.6% Cys (C) 6 1.3% Gln (Q) 17 3.8% Glu (E) 12 2.7% Gly (G) 30 6.7% His (H) 11 2.5% Ile (I) 37 8.3% Leu (L) 52 11.6% Lys (K) 27 6.0% Met (M) 14 3.1% Phe (F) 39 8.7% Pro (P) 17 3.8% Ser (S) 31 6.9% Thr (T) 23 5.1% Trp (W) 12 2.7% Tyr (Y) 15 3.4% Val (V) 32 7.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28 Total number of positively charged residues (Arg + Lys): 43 Atomic composition: Carbon C 2396 Hydrogen H 3662 Nitrogen N 586 Oxygen O 603 Sulfur S 20 Formula: C2396H3662N586O603S20 Total number of atoms: 7267 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 88725 Abs 0.1% (=1 g/l) 1.741, assuming all pairs of Cys residues form cystines Ext. coefficient 88350 Abs 0.1% (=1 g/l) 1.734, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 31.06 This classifies the protein as stable. Aliphatic index: 104.45 Grand average of hydropathicity (GRAVY): 0.385