ProtParam
User-provided sequence:
10 20 30 40 50 60
MLDVLKFNFK FSQVVSVFVS NSSNMQKEIA GDAPSAPTTR VRHRKRNSDV GAGAGKPNGN
70 80 90 100 110 120
HLLVNDSKKY KSFLIRAYST FWMIGGFALI VYLGHLYITA MVVVIQIFMA RELFNLLRKT
130 140 150 160 170 180
HEDKQLPGFR LLNWHFFFTA MLFVYGRILS QRLVNTVTPD KVLYRLVTSL IKYHMAICYS
190 200 210 220 230 240
LYISGFVWFI LTLKKKMYKY QFSQYAWTHM ILIVVFTQSS FTVANIFEGI FWFLLPASLI
250 260 270 280 290 300
VINDIFAYIC GFFFGRTPLI KLSPKKTWEG FIGASITTVI SAFLLANIMG RFLWLTCPRE
310 320 330 340 350 360
DLSTGWLLCD PGPLFKQETH ALPGWISDWL PWKEISILPV QWHALCLGLF ASIIAPFGGF
370 380 390 400 410 420
FASGFKRAFK IKDFGDSIPG HGGITDRMDC QMVMAVFAYI YHQSFVVPEV LSVDKLLDQI
430 440
ITSLTLEEQQ ALLVKLGQML QEKVIGS
References and
documentation are available.
Number of amino acids: 447
Molecular weight: 50966.20
Theoretical pI: 9.47
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 2396
Hydrogen H 3662
Nitrogen N 586
Oxygen O 603
Sulfur S 20
Formula: C
2396H
3662N
586O
603S
20
Total number of atoms: 7267
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 88725
Abs 0.1% (=1 g/l) 1.741, assuming all pairs of Cys residues form cystines
Ext. coefficient 88350
Abs 0.1% (=1 g/l) 1.734, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.06
This classifies the protein as stable.
Aliphatic index: 104.45
Grand average of hydropathicity (GRAVY): 0.385