ProtParam
User-provided sequence:
10 20 30 40 50 60
MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL EIGVEAFQEY
70 80 90 100 110 120
RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII LLFEDTKKDR IAEYSLKLMD
130 140 150 160 170 180
IDADFLKIEE LQYDSTLSLP SSEFSKIVRD LSQLSDSINI MITKETIKFV ADGDIGSGSV
190 200 210 220 230 240
IIKPFVDMEH PETSIKLEMD QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD
250
LKSGFLQFFL APKFNDEE
References and
documentation are available.
Number of amino acids: 258
Molecular weight: 28916.10
Theoretical pI: 4.42
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1295
Hydrogen H 2061
Nitrogen N 319
Oxygen O 406
Sulfur S 10
Formula: C
1295H
2061N
319O
406S
10
Total number of atoms: 4091
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6210
Abs 0.1% (=1 g/l) 0.215, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.206, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.42
This classifies the protein as stable.
Aliphatic index: 104.30
Grand average of hydropathicity (GRAVY): 0.000