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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL EIGVEAFQEY

70 80 90 100 110 120
RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII LLFEDTKKDR IAEYSLKLMD

130 140 150 160 170 180
IDADFLKIEE LQYDSTLSLP SSEFSKIVRD LSQLSDSINI MITKETIKFV ADGDIGSGSV

190 200 210 220 230 240
IIKPFVDMEH PETSIKLEMD QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD

250
LKSGFLQFFL APKFNDEE


References and documentation are available.

Number of amino acids: 258 Molecular weight: 28916.10 Theoretical pI: 4.42
Amino acid composition: 
Ala (A) 13 5.0% Arg (R) 8 3.1% Asn (N) 6 2.3% Asp (D) 27 10.5% Cys (C) 4 1.6% Gln (Q) 9 3.5% Glu (E) 19 7.4% Gly (G) 12 4.7% His (H) 2 0.8% Ile (I) 24 9.3% Leu (L) 32 12.4% Lys (K) 18 7.0% Met (M) 6 2.3% Phe (F) 17 6.6% Pro (P) 8 3.1% Ser (S) 24 9.3% Thr (T) 12 4.7% Trp (W) 0 0.0% Tyr (Y) 4 1.6% Val (V) 13 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46 Total number of positively charged residues (Arg + Lys): 26 Atomic composition: Carbon C 1295 Hydrogen H 2061 Nitrogen N 319 Oxygen O 406 Sulfur S 10 Formula: C1295H2061N319O406S10 Total number of atoms: 4091 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 6210 Abs 0.1% (=1 g/l) 0.215, assuming all pairs of Cys residues form cystines Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.206, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 27.42 This classifies the protein as stable. Aliphatic index: 104.30 Grand average of hydropathicity (GRAVY): 0.000