ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLEGKAMVED SDMPVKMQMQ AMAFASQALD LFDVFDCKSI AGHIKKEFDE RYGSGWQCVV 

        70         80         90 
GSNFGCFFTH SKGTFIYFQL ETLKFLIFKG ASTP 

References and documentation are available.

Number of amino acids: 94

Molecular weight: 10582.25

Theoretical pI: 5.54

Amino acid composition: 
Ala (A)   7	  7.4%
Arg (R)   1	  1.1%
Asn (N)   1	  1.1%
Asp (D)   6	  6.4%
Cys (C)   3	  3.2%
Gln (Q)   5	  5.3%
Glu (E)   5	  5.3%
Gly (G)   8	  8.5%
His (H)   2	  2.1%
Ile (I)   4	  4.3%
Leu (L)   6	  6.4%
Lys (K)   8	  8.5%
Met (M)   6	  6.4%
Phe (F)  11	 11.7%
Pro (P)   2	  2.1%
Ser (S)   7	  7.4%
Thr (T)   4	  4.3%
Trp (W)   1	  1.1%
Tyr (Y)   2	  2.1%
Val (V)   5	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 9

Atomic composition:

Carbon      C	       480
Hydrogen    H	       722
Nitrogen    N	       116
Oxygen      O	       136
Sulfur      S	         9

Formula: C480H722N116O136S9
Total number of atoms: 1463

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8605
Abs 0.1% (=1 g/l)   0.813, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.801, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.14
This classifies the protein as stable.



Aliphatic index: 64.36

Grand average of hydropathicity (GRAVY): 0.045