ProtParam
User-provided sequence:
10 20 30 40 50 60
MLEGKAVMGD TDMKQTMKED ALSLASKALD CFDVTEPTQI ARFIKKEFDR SYGSGWQCIV
70 80 90 100
GTHFGSFVTH CSGCFIHFSV GSLTILLFKG SVGEPAPPDR CSS
References and
documentation are available.
Number of amino acids: 103
Molecular weight: 11201.84
Theoretical pI: 5.83
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 10
Atomic composition:
Carbon C 494
Hydrogen H 767
Nitrogen N 129
Oxygen O 150
Sulfur S 9
Formula: C
494H
767N
129O
150S
9
Total number of atoms: 1549
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7240
Abs 0.1% (=1 g/l) 0.646, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 0.624, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 19.70
This classifies the protein as stable.
Aliphatic index: 68.16
Grand average of hydropathicity (GRAVY): -0.016