ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLEIGFCTLE DQCPYLKDKR SRIEYNYIEN CPKEINNELI KRGWRRFGRY FSRPICKDCD 

        70         80         90        100        110        120 
ECLSLRILVN EYNFSRSERR VVNKNINTKV ILRTPNLSNE HLFLYDKYHR FMEEKKNWKR 

       130        140        150        160        170        180 
YDLSFKQYYN LYVDGFMNFG YELAFYIEDK LVCVDLIDIL EDGISSIYCF YDPDFSYFSL 

       190        200        210        220        230 
GKFSLLNEIQ IAKKMNLDYI YLGYFVKKCQ SLSYKADYTP NEILKGTKEL FENEVLWEK 

References and documentation are available.

Number of amino acids: 239

Molecular weight: 29001.31

Theoretical pI: 6.74

Amino acid composition: 
Ala (A)   3	  1.3%
Arg (R)  14	  5.9%
Asn (N)  18	  7.5%
Asp (D)  15	  6.3%
Cys (C)   9	  3.8%
Gln (Q)   4	  1.7%
Glu (E)  21	  8.8%
Gly (G)   9	  3.8%
His (H)   2	  0.8%
Ile (I)  18	  7.5%
Leu (L)  26	 10.9%
Lys (K)  22	  9.2%
Met (M)   4	  1.7%
Phe (F)  16	  6.7%
Pro (P)   6	  2.5%
Ser (S)  14	  5.9%
Thr (T)   5	  2.1%
Trp (W)   3	  1.3%
Tyr (Y)  21	  8.8%
Val (V)   9	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1326
Hydrogen    H	      2008
Nitrogen    N	       332
Oxygen      O	       374
Sulfur      S	        13

Formula: C1326H2008N332O374S13
Total number of atoms: 4053

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48290
Abs 0.1% (=1 g/l)   1.665, assuming all pairs of Cys residues form cystines


Ext. coefficient    47790
Abs 0.1% (=1 g/l)   1.648, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.03
This classifies the protein as unstable.



Aliphatic index: 83.97

Grand average of hydropathicity (GRAVY): -0.495