ProtParam
User-provided sequence:
10 20 30 40 50 60
MLEQVEQKNQ EQKKPQKFNV FPKIINGGVA GIIGVACVYP LDMVKTRLQN QTIGPNGERM
70 80 90 100 110 120
YTSIADCFRK TIASEGYFGM YRGSAVNIVL ITPEKAIKLT ANDFFRYHLA SDDGVIPLSR
130 140 150 160 170 180
ATLAGGLAGL FQIVVTTPME LLKIQMQDAG RVAAADRAAG REVKTITALG LTKTLLRERG
190 200 210 220 230 240
IFGLYKGVGA TGVRDITFSM VYFPLMAWIN DQGPRKSDGS GEAVFYWSLI AGLLSGMTSA
250 260 270 280 290 300
FMVTPFDVVK TRLQADGEKK FKGIMDCVNR TLKEEGISAF FKGGLCRIMV LAPLFGIAQM
310
FYFLGVGEKI LGIERTKSV
References and
documentation are available.
Number of amino acids: 319
Molecular weight: 34912.93
Theoretical pI: 9.46
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1576
Hydrogen H 2524
Nitrogen N 418
Oxygen O 440
Sulfur S 17
Formula: C
1576H
2524N
418O
440S
17
Total number of atoms: 4975
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24660
Abs 0.1% (=1 g/l) 0.706, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.699, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 17.09
This classifies the protein as stable.
Aliphatic index: 93.86
Grand average of hydropathicity (GRAVY): 0.131