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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLEQVEQKNQ EQKKPQKFNV FPKIINGGVA GIIGVACVYP LDMVKTRLQN QTIGPNGERM

70 80 90 100 110 120
YTSIADCFRK TIASEGYFGM YRGSAVNIVL ITPEKAIKLT ANDFFRYHLA SDDGVIPLSR

130 140 150 160 170 180
ATLAGGLAGL FQIVVTTPME LLKIQMQDAG RVAAADRAAG REVKTITALG LTKTLLRERG

190 200 210 220 230 240
IFGLYKGVGA TGVRDITFSM VYFPLMAWIN DQGPRKSDGS GEAVFYWSLI AGLLSGMTSA

250 260 270 280 290 300
FMVTPFDVVK TRLQADGEKK FKGIMDCVNR TLKEEGISAF FKGGLCRIMV LAPLFGIAQM

310
FYFLGVGEKI LGIERTKSV


References and documentation are available.

Number of amino acids: 319 Molecular weight: 34912.93 Theoretical pI: 9.46
Amino acid composition: 
Ala (A) 27 8.5% Arg (R) 17 5.3% Asn (N) 9 2.8% Asp (D) 13 4.1% Cys (C) 4 1.3% Gln (Q) 13 4.1% Glu (E) 15 4.7% Gly (G) 35 11.0% His (H) 1 0.3% Ile (I) 24 7.5% Leu (L) 28 8.8% Lys (K) 22 6.9% Met (M) 13 4.1% Phe (F) 19 6.0% Pro (P) 11 3.4% Ser (S) 13 4.1% Thr (T) 20 6.3% Trp (W) 2 0.6% Tyr (Y) 9 2.8% Val (V) 24 7.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28 Total number of positively charged residues (Arg + Lys): 39 Atomic composition: Carbon C 1576 Hydrogen H 2524 Nitrogen N 418 Oxygen O 440 Sulfur S 17 Formula: C1576H2524N418O440S17 Total number of atoms: 4975 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 24660 Abs 0.1% (=1 g/l) 0.706, assuming all pairs of Cys residues form cystines Ext. coefficient 24410 Abs 0.1% (=1 g/l) 0.699, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 17.09 This classifies the protein as stable. Aliphatic index: 93.86 Grand average of hydropathicity (GRAVY): 0.131