ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLETNVDQKS FKFKIVLLGE GCVGKSSLVL RFVENKFSCK HLSTIQASFQ NKTVNVEDCQ 

        70         80         90        100        110        120 
ADLHIWDTAG QEKYHALGPI YYRGSNGVLL VFDITDRKSF EKVKNWVLEI KTCLGNTAEI 

       130        140        150        160        170        180 
LIVGNKIDLE EERQVTRQDA EAYAESEGAL YMETSAQDNV GISDAFESLT AKMIEHSRTR 

       190        200 
STEPPSTNRS IRLIDNDEAE RSKKCCR 

References and documentation are available.

Number of amino acids: 207

Molecular weight: 23377.40

Theoretical pI: 5.51

Amino acid composition: 
Ala (A)  13	  6.3%
Arg (R)  11	  5.3%
Asn (N)  11	  5.3%
Asp (D)  12	  5.8%
Cys (C)   6	  2.9%
Gln (Q)   8	  3.9%
Glu (E)  20	  9.7%
Gly (G)  11	  5.3%
His (H)   4	  1.9%
Ile (I)  13	  6.3%
Leu (L)  17	  8.2%
Lys (K)  16	  7.7%
Met (M)   3	  1.4%
Phe (F)   8	  3.9%
Pro (P)   3	  1.4%
Ser (S)  17	  8.2%
Thr (T)  13	  6.3%
Trp (W)   2	  1.0%
Tyr (Y)   5	  2.4%
Val (V)  14	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1019
Hydrogen    H	      1629
Nitrogen    N	       285
Oxygen      O	       326
Sulfur      S	         9

Formula: C1019H1629N285O326S9
Total number of atoms: 3268

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18825
Abs 0.1% (=1 g/l)   0.805, assuming all pairs of Cys residues form cystines


Ext. coefficient    18450
Abs 0.1% (=1 g/l)   0.789, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.44
This classifies the protein as stable.



Aliphatic index: 82.42

Grand average of hydropathicity (GRAVY): -0.459