ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLFARLVLLL VYLAPGSLAK PASTKKRTQW DQIAIDACAK ELESHKFDTD VKGRHATLCT 

        70         80         90        100        110        120 
YEPALGSWLH CAKDVLDSRK KSKKIFEKTF SKINQYCHDY HKDEVVSNEE YYRIFANASL 

       130        140        150        160        170        180 
FIRPLDEVKE NIRYPVTPNK ASLDRWVWAY FGPLDNIDKG NVYGVTICLY WIGVLFIAAV 

       190        200        210        220        230        240 
YHFLNFSRLK QTVFKNKVSA FLRGHYVLPA LVHNHAMSVG RWFFIGLVPT RLETLVLFGY 

       250        260        270        280        290        300 
VLLHGFLLSS YNFDHNELLS DRRSQVLIFL SDRAGILAFA HFPLIVLFGG KNSTMTWLTG 

       310        320        330        340        350        360 
IRYTAFITYH KWLGRFMLVD CTIHAIGYTY HAYIENYWKY VKYSDLWTSG RHAMIIVGIL 

       370        380        390        400        410        420 
VFFSFFFFRR HYYELFVITH IILAIGFFHA CWKHCYKLGW GEWIMACALF WIADRILRLI 

       430        440        450        460        470        480 
KIAIFGMPWA KLKLCGESMI EVRISKSSKW WKAEPGQYIY LYFLRPKIFW QSHPFTVMDS 

       490        500        510        520        530        540 
LVEDGELVVV ITVKNGLTKK LQEYLLESEG YTEMRVLAEG PYGQSTRTHL FESLLFIAGG 

       550        560        570        580        590        600 
AGVPGPLSMA IKAGRQVKSN DSHQMIKFVW SVRNLDLLEV YRKEIMVLKE LNIDTKIYFT 

       610        620        630        640        650        660 
GERKDESNTE EGAIANMSTE GRLLTTSKSA EMITDFGRPN IDEIIEEAVS GAKSLLVTCC 

       670        680        690 
GSEGFVDKTR ELTAKRVLEH GDKWIEYVEE FQNW 

References and documentation are available.

Number of amino acids: 694

Molecular weight: 80292.37

Theoretical pI: 8.83

Amino acid composition: 
Ala (A)  45	  6.5%
Arg (R)  34	  4.9%
Asn (N)  22	  3.2%
Asp (D)  28	  4.0%
Cys (C)  12	  1.7%
Gln (Q)  12	  1.7%
Glu (E)  43	  6.2%
Gly (G)  44	  6.3%
His (H)  24	  3.5%
Ile (I)  50	  7.2%
Leu (L)  75	 10.8%
Lys (K)  48	  6.9%
Met (M)  15	  2.2%
Phe (F)  44	  6.3%
Pro (P)  18	  2.6%
Ser (S)  42	  6.1%
Thr (T)  36	  5.2%
Trp (W)  21	  3.0%
Tyr (Y)  34	  4.9%
Val (V)  47	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      3723
Hydrogen    H	      5673
Nitrogen    N	       947
Oxygen      O	       983
Sulfur      S	        27

Formula: C3723H5673N947O983S27
Total number of atoms: 11353

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   166910
Abs 0.1% (=1 g/l)   2.079, assuming all pairs of Cys residues form cystines


Ext. coefficient   166160
Abs 0.1% (=1 g/l)   2.069, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.32
This classifies the protein as stable.



Aliphatic index: 96.37

Grand average of hydropathicity (GRAVY): 0.025