ProtParam
User-provided sequence:
10 20 30 40 50 60
MLFFILFLSI ISPIESVDQR IHHPSKCDHL SKFPTKGFTM VLKVSLNGCG RFKRVQDAID
70 80 90 100 110 120
ASIGSSQSKT LILIDFGIYR ERFIVHENKN NLVVQGMGYS RTSIEWNNTT ASSNGTFSSF
130 140 150 160 170 180
SVAVFGEKFT AYNISFKNTA PAPNPGAVDA QAVALKVVGD KAAFYGCGFY GNQDTLLDQE
190 200 210 220 230 240
GRHFFKGCFI EGSIDFIFGN GRSLYEDCTL HSIAKENTIG CITANGKDTL KDRTGFVFVN
250 260 270 280 290 300
CKITGSARVW LGRAWRPYAR VIFSKTYMSR VVSLDGWNDM GDPKTQRTVY YGEHRCYGPG
310 320 330
ANHSKRVTYA KLLSDVEAAP FTNISFIDGE EWL
References and
documentation are available.
Number of amino acids: 333
Molecular weight: 37067.12
Theoretical pI: 8.80
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1668
Hydrogen H 2551
Nitrogen N 451
Oxygen O 483
Sulfur S 13
Formula: C
1668H
2551N
451O
483S
13
Total number of atoms: 5166
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45880
Abs 0.1% (=1 g/l) 1.238, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.224, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.90
This classifies the protein as stable.
Aliphatic index: 76.13
Grand average of hydropathicity (GRAVY): -0.162