ProtParam
User-provided sequence:
10 20 30 40 50 60
MLFFLIFLGL MATASAKAGR CNEGDQDAHF DSNKFWKCEG GRWIVVTCQI NYFYDATTGF
70 80 90 100 110 120
CYLSRFPTTR PYPPPFPTRP RPTQGHRCYP GTRIPYPHDH TMFLECSTDG TEYIKRYCQP
130 140 150 160 170 180
DAVFVQRENM CVAIQTTYRP TRTWPTTTTT PYYGACKESA GHDGYKPDVY NCKNFYQCAS
190 200 210 220 230
GLWTQRSCGE GTIWDQHKLT CDHNRGQCRP TSPTVQPTYQ PTWRPTAYPP NEVKK
References and
documentation are available.
Number of amino acids: 235
Molecular weight: 27089.55
Theoretical pI: 8.71
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1207
Hydrogen H 1785
Nitrogen N 333
Oxygen O 347
Sulfur S 18
Formula: C
1207H
1785N
333O
347S
18
Total number of atoms: 3690
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57715
Abs 0.1% (=1 g/l) 2.131, assuming all pairs of Cys residues form cystines
Ext. coefficient 56840
Abs 0.1% (=1 g/l) 2.098, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.28
This classifies the protein as stable.
Aliphatic index: 39.87
Grand average of hydropathicity (GRAVY): -0.735