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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLFFSFFKTL VDQEVVVELK NDIEIKGTLQ SVDQFLNLKL DNISCTDEKK YPHLGSVRNI

70 80 90
FIRGSTVRYV YLNKNMVDTN LLQDATRREV MTERK


References and documentation are available.

Number of amino acids: 95 Molecular weight: 11162.90 Theoretical pI: 7.84
Amino acid composition: 
Ala (A) 1 1.1% Arg (R) 6 6.3% Asn (N) 7 7.4% Asp (D) 7 7.4% Cys (C) 1 1.1% Gln (Q) 4 4.2% Glu (E) 6 6.3% Gly (G) 3 3.2% His (H) 1 1.1% Ile (I) 5 5.3% Leu (L) 11 11.6% Lys (K) 8 8.4% Met (M) 3 3.2% Phe (F) 6 6.3% Pro (P) 1 1.1% Ser (S) 5 5.3% Thr (T) 7 7.4% Trp (W) 0 0.0% Tyr (Y) 3 3.2% Val (V) 10 10.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 13 Total number of positively charged residues (Arg + Lys): 14 Atomic composition: Carbon C 498 Hydrogen H 802 Nitrogen N 134 Oxygen O 148 Sulfur S 4 Formula: C498H802N134O148S4 Total number of atoms: 1586 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.400, assuming all pairs of Cys residues form cystines Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.400, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 42.92 This classifies the protein as unstable. Aliphatic index: 97.26 Grand average of hydropathicity (GRAVY): -0.294