ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLFRNIEVGR QAAKLLTRTS SRLAWQSIGA SRNISTIRQQ IRKTQLYNFK KTVSIRPFSL 

        70         80         90        100        110        120 
SSPVFKPHVA SESNPIESRL KTSKNVAYWL IGTSGLVFGI VVLGGLTRLT ESGLSITEWK 

       130        140        150        160        170        180 
PVTGTLPPMN QKEWEEEFIK YKESPEFKLL NSHIDLDEFK FIFFMEWIHR LWGRAIGAVF 

       190        200        210        220        230        240 
ILPAVYFAVS KKTSGHVNKR LFGLAGLLGL QGFVGWWMVK SGLDQEQLDA RKSKPTVSQY 

       250        260        270        280        290        300 
RLTTHLGTAF FLYMGMLWTG LEILRECKWI KNPVQAISLF KKLDNPAIGP MRKISLALLA 

       310        320        330        340        350        360 
VSFLTAMSGG MVAGLDAGWV YNTWPKMGER WFPSSRELMD ENFCRREDKK DLWWRNLLEN 

       370        380        390        400        410        420 
PVTVQLVHRT CAYVAFTSVL AAHMYAIKKK AVIPRNAMTS LHVMMGVVTL QATLGILTIL 

       430        440        450        460        470        480 
YLVPISLASI HQAGALALLT SSLVFASQLR KPRAPMRNVI ITLPHSSKVT SGKILSEASK 


LASKPL 

References and documentation are available.

Number of amino acids: 486

Molecular weight: 54658.39

Theoretical pI: 10.17

Amino acid composition: 
Ala (A)  36	  7.4%
Arg (R)  27	  5.6%
Asn (N)  16	  3.3%
Asp (D)   9	  1.9%
Cys (C)   3	  0.6%
Gln (Q)  15	  3.1%
Glu (E)  22	  4.5%
Gly (G)  31	  6.4%
His (H)  10	  2.1%
Ile (I)  30	  6.2%
Leu (L)  61	 12.6%
Lys (K)  35	  7.2%
Met (M)  16	  3.3%
Phe (F)  23	  4.7%
Pro (P)  22	  4.5%
Ser (S)  42	  8.6%
Thr (T)  30	  6.2%
Trp (W)  15	  3.1%
Tyr (Y)  10	  2.1%
Val (V)  33	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2505
Hydrogen    H	      3982
Nitrogen    N	       668
Oxygen      O	       662
Sulfur      S	        19

Formula: C2505H3982N668O662S19
Total number of atoms: 7836

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    97525
Abs 0.1% (=1 g/l)   1.784, assuming all pairs of Cys residues form cystines


Ext. coefficient    97400
Abs 0.1% (=1 g/l)   1.782, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.02
This classifies the protein as stable.



Aliphatic index: 100.12

Grand average of hydropathicity (GRAVY): 0.076