ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLFSWPYPEA PIEGYWGKPT SLIDWCEENY VVSPYIAEWS NTITNSIFLM TAFYSTYSAW 

        70         80         90        100        110        120 
RNKLETRYIL IGMGFSLVGI GSWLFHMTLQ YRYQLLDELP MLYATIIPSW SIFAETQEIL 

       130        140        150        160        170        180 
IKDEKKRKES SFRIQMVISF IMCGIVTILT WIYVVVQKPA IFQVLYGILT LLVVVLSGWL 

       190        200        210        220        230        240 
TYYHVHDSFA KKNLFITMVM GMIPFVIGFI CWQLDIHLCS FWIYIRRTYL ALPLGVLLEL 

       250        260        270        280        290        300 
HAWWHLLTGT GVYIFVVYLQ YLRILTHGNP NDFLFIWRWG FFPELVRKGL PIGTSYSLEY 

       310 
LGPIVNTQVD DETKKNN 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 37230.85

Theoretical pI: 6.65

Amino acid composition: 
Ala (A)  10	  3.2%
Arg (R)  10	  3.2%
Asn (N)  10	  3.2%
Asp (D)   8	  2.5%
Cys (C)   4	  1.3%
Gln (Q)   9	  2.8%
Glu (E)  15	  4.7%
Gly (G)  19	  6.0%
His (H)   7	  2.2%
Ile (I)  33	 10.4%
Leu (L)  39	 12.3%
Lys (K)  12	  3.8%
Met (M)  10	  3.2%
Phe (F)  19	  6.0%
Pro (P)  14	  4.4%
Ser (S)  19	  6.0%
Thr (T)  21	  6.6%
Trp (W)  15	  4.7%
Tyr (Y)  21	  6.6%
Val (V)  22	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1774
Hydrogen    H	      2649
Nitrogen    N	       407
Oxygen      O	       444
Sulfur      S	        14

Formula: C1774H2649N407O444S14
Total number of atoms: 5288

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   114040
Abs 0.1% (=1 g/l)   3.063, assuming all pairs of Cys residues form cystines


Ext. coefficient   113790
Abs 0.1% (=1 g/l)   3.056, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.36
This classifies the protein as stable.



Aliphatic index: 111.86

Grand average of hydropathicity (GRAVY): 0.402