ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLGEDEGNTV LEKGNNPSVK QGEVGAVFIV PKILIREHER VILKQILQIL DQDELVQPPL 

        70         80         90        100        110        120 
DKFPYKKLEL PKYIDELKTR DATNTSYKMI QLDAYGEKKV GSNGELFGGR HYLFNTFTFT 

       130        140        150        160        170        180 
AHMGVLLVLL QDVIKVLYQS NATHDEDEFI VQHDQILVME TSEEQTKFLA KNGVIPEESK 

       190        200        210        220        230        240 
GSFKYITARS AFVEFGASVI AGGQRIVDDY WESLAKKQNL SSHQRVFKLS TNLISKISLL 

       250        260        270        280        290        300 
RPSFQNNRIS NANEISANTN NTCTISTSKF ESQYPIVTEQ PSAEIREAYI ENFAKGEHIS 

       310        320        330        340        350        360 
AIVPGQSISG TLELSAQFRV PRYHSKNSFQ QALQMKAMDI PIGRHEELLA QYESQAPDGS 

       370        380        390        400        410        420 
ASISLPNHIP SVNPSNKPIK RMLSSILDIN VSSSKNKKSE ENEMIKPMNK GQHKNNTSLN 

       430        440        450        460        470        480 
INGWKFESLP LKSAENSGKQ QYYRGLPLYE KNTLLERLKQ LTPNEIKELE HLHDAVFVNT 

       490        500        510        520        530        540 
GLQNVRKVRT KKWKKYWQYK AGIPIGLKRS QLDEFKNKYL KDVLAQTSVT TNFNEITNTD 

       550        560        570        580        590        600 
ETITTKRVPN PNFLGNCNIK DFKPPYIYSH VNKVPQNVAG DKTAVKLDTE VKNTNANPVV 

       610        620 
ATDPVAAKPD NLANFSNEVA MNN 

References and documentation are available.

Number of amino acids: 623

Molecular weight: 70275.79

Theoretical pI: 8.77

Amino acid composition: 
Ala (A)  35	  5.6%
Arg (R)  20	  3.2%
Asn (N)  53	  8.5%
Asp (D)  25	  4.0%
Cys (C)   2	  0.3%
Gln (Q)  33	  5.3%
Glu (E)  46	  7.4%
Gly (G)  30	  4.8%
His (H)  14	  2.2%
Ile (I)  42	  6.7%
Leu (L)  54	  8.7%
Lys (K)  57	  9.1%
Met (M)  10	  1.6%
Phe (F)  24	  3.9%
Pro (P)  31	  5.0%
Ser (S)  48	  7.7%
Thr (T)  35	  5.6%
Trp (W)   4	  0.6%
Tyr (Y)  20	  3.2%
Val (V)  40	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      3129
Hydrogen    H	      4972
Nitrogen    N	       858
Oxygen      O	       955
Sulfur      S	        12

Formula: C3129H4972N858O955S12
Total number of atoms: 9926

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51925
Abs 0.1% (=1 g/l)   0.739, assuming all pairs of Cys residues form cystines


Ext. coefficient    51800
Abs 0.1% (=1 g/l)   0.737, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.21
This classifies the protein as stable.



Aliphatic index: 84.33

Grand average of hydropathicity (GRAVY): -0.553