ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLGIRSSVKT CFKPMSLTSK RLISQSLLAS KSTYRTPNFD DVLKENNDAD KGRSYAYFMV 

        70         80         90        100        110        120 
GAMGLLSSAG AKSTVETFIS SMTATADVLA MAKVEVNLAA IPLGKNVVVK WQGKPVFIRH 

       130        140        150        160        170        180 
RTPHEIQEAN SVDMSALKDP QTDADRVKDP QWLIMLGICT HLGCVPIGEA GDFGGWFCPC 

       190        200        210 
HGSHYDISGR IRKGPAPLNL EIPAYEFDGD KVIVG 

References and documentation are available.

Number of amino acids: 215

Molecular weight: 23364.95

Theoretical pI: 8.24

Amino acid composition: 
Ala (A)  18	  8.4%
Arg (R)   9	  4.2%
Asn (N)   7	  3.3%
Asp (D)  14	  6.5%
Cys (C)   5	  2.3%
Gln (Q)   5	  2.3%
Glu (E)   8	  3.7%
Gly (G)  18	  8.4%
His (H)   5	  2.3%
Ile (I)  13	  6.0%
Leu (L)  17	  7.9%
Lys (K)  15	  7.0%
Met (M)   8	  3.7%
Phe (F)   8	  3.7%
Pro (P)  12	  5.6%
Ser (S)  18	  8.4%
Thr (T)  11	  5.1%
Trp (W)   3	  1.4%
Tyr (Y)   5	  2.3%
Val (V)  16	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1036
Hydrogen    H	      1646
Nitrogen    N	       282
Oxygen      O	       306
Sulfur      S	        13

Formula: C1036H1646N282O306S13
Total number of atoms: 3283

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24200
Abs 0.1% (=1 g/l)   1.036, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   1.025, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.09
This classifies the protein as stable.



Aliphatic index: 84.37

Grand average of hydropathicity (GRAVY): -0.088