ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLGVEKPYVV EPAMAVAQDS FEFGSNGDGS STNSHTSNAS SKDLGRLTSG DGAQNFAQDS 

        70         80         90        100        110        120 
SANSIDQDST DNTNSDCNPI TLNDLLGLCG ASTANEVVGT GQTPSTSAPI LGAGGGGRAN 

       130        140        150        160        170        180 
GVTAGAATAT GVGVGAGGTL PGPGVPSIQG GGGGGVVGQQ TNASCNTSAA NPSASFGGNG 

       190        200        210        220        230        240 
SSSDVNNLLL ASAAAAAAAA AGDGAQLSAN AAAYAPLTPS STHSSASPGT KSNFDYFQFE 

       250        260        270        280        290        300 
NVAQSNPLKA FQRTNISFDC SAPLSPSTPT SIYNRSFHSS PLVSDSSNSS SGIGLSMDSI 

       310        320        330        340        350        360 
NMFYNQQQQQ QQPEQQGYTS LGNSMGSGLG LSLANASTRS NSPESQNSSN STTEQNLLDM 

       370        380        390        400        410        420 
INLLSVNSNK IPHQQQQQQQ QQQQQQQQQQ QNQQQLQVQQ QHQLQQQFVN LNRNYEQQIS 

       430        440        450        460        470        480 
ANLGSQQHGF EHNGVGVGAS SSGNENCFSQ YNLENITSVD MELAKLQNLQ RINTLKLLHA 

       490        500        510        520        530        540 
QAQQMPLINQ LLQSYAGNAI GSVGGSNLGN LMSAGGSSLM TEMAGNVGGI ITTNDGHLDR 

       550        560        570        580        590        600 
VAKFYKSSAA LCDATCTWSG HLPPRSHRML NYSPKVFLGG IPWDISEQSL IQIFKPFGSI 

       610        620        630        640        650        660 
KVEWPGKEQQ AAQPKGYVYI IFESDKQVKA LLSACVLQVD DSHCGRNYFF KISSRRIKSK 

       670        680        690        700        710        720 
DVEVIPWIIA DSNFVRSSSQ KLDPTKTVFV GALHGKLTAE GLGNIMDDLF DGVLYAGIDT 

       730        740        750        760        770        780 
DKYKYPIGSG RVTFSNFRSY MKAVSAAFIE IRTTKFTKKV QVDPYLEDAL CSICGVQHGP 

       790        800        810        820        830        840 
YYCRELSCFR YFCRSCWQWQ HSCDIVKNHK PLTRNSKSQS LVGIGPSSSN VSLPFSGQRS 

       850        860        870        880        890        900 
IRDNRMGNGQ HQQHQQHQYQ QQKHRQLQEY SQPHSLNVMG NSGAANAAAT SMVTLQQRQI 

       910 
HKVRIQRQQH QAI 

References and documentation are available.

Number of amino acids: 913

Molecular weight: 97670.98

Theoretical pI: 7.30

Amino acid composition: 
Ala (A)  74	  8.1%
Arg (R)  28	  3.1%
Asn (N)  66	  7.2%
Asp (D)  37	  4.1%
Cys (C)  16	  1.8%
Gln (Q)  97	 10.6%
Glu (E)  24	  2.6%
Gly (G)  86	  9.4%
His (H)  23	  2.5%
Ile (I)  41	  4.5%
Leu (L)  67	  7.3%
Lys (K)  33	  3.6%
Met (M)  17	  1.9%
Phe (F)  31	  3.4%
Pro (P)  37	  4.1%
Ser (S) 115	 12.6%
Thr (T)  44	  4.8%
Trp (W)   6	  0.7%
Tyr (Y)  24	  2.6%
Val (V)  47	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      4198
Hydrogen    H	      6589
Nitrogen    N	      1245
Oxygen      O	      1382
Sulfur      S	        33

Formula: C4198H6589N1245O1382S33
Total number of atoms: 13447

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69760
Abs 0.1% (=1 g/l)   0.714, assuming all pairs of Cys residues form cystines


Ext. coefficient    68760
Abs 0.1% (=1 g/l)   0.704, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.37
This classifies the protein as unstable.



Aliphatic index: 69.17

Grand average of hydropathicity (GRAVY): -0.478