ProtParam
User-provided sequence:
10 20 30 40 50 60
MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK
70 80 90 100 110 120
EYLPETSLAP PGQSKVDLLQ GSQAGSKIKP TAEQLAQWEA LKSVPIPPRK NATLDHITNM
130 140 150 160 170 180
IMRHGKKEKA QTILSRALYL VYCQTRQDPI QALEKSLDEL APLMMTKTFN TGVAKASVIP
190 200 210 220 230 240
VPLNKRQRNR IAWNWIVQSA NQRVSSDFAV RLGEELTAIA KGTSSAFEKR DQIHKTAIAH
RAYIQLK
References and
documentation are available.
Number of amino acids: 247
Molecular weight: 27816.10
Theoretical pI: 9.76
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1230
Hydrogen H 1996
Nitrogen N 358
Oxygen O 358
Sulfur S 9
Formula: C
1230H
1996N
358O
358S
9
Total number of atoms: 3951
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35075
Abs 0.1% (=1 g/l) 1.261, assuming all pairs of Cys residues form cystines
Ext. coefficient 34950
Abs 0.1% (=1 g/l) 1.256, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.21
This classifies the protein as unstable.
Aliphatic index: 86.19
Grand average of hydropathicity (GRAVY): -0.433