ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLHELLLALL GFTGDLIVDE REQRKTLGLA FNSDSPLSDE CTFKLAPDIS FIEPSERDLI 

        70         80         90        100        110        120 
ERLIKLGFYY RELDRFAKKS RNLSWIRSVT SVHPLERADE LSKQSREKKP SVYRRAIANG 

       130        140        150        160        170        180 
IGEILSVYRS AVLHIEQKLL AETTPILATV TEGLNKFFVL FPPLYEVILE IERDDIRGGQ 

       190        200        210        220        230        240 
LLNVLNKRCH CGVPELRTCL QRLLWNGHQV MYNQLAAWMV YGILQDPHGE FFIKRQDDGD 

       250        260        270        280        290        300 
LDHRSSQEEV SEKLARTSVH ETSLTDWHSG FHISLDMLPD YIPMRLGESI LFAGKAIRVL 

       310        320        330        340        350        360 
RNPSPAFQFQ KDKSFQQTMR GSQRIRGFMH SDFPETETEL DADLTGGELL PQSEADKIEA 

       370        380        390        400        410        420 
MLKDLKESSE FHKRSFECTV DSVRAIAASH LWQLVVVRAD LNGHLKALKD YFLLEKGDFF 

       430        440        450        460        470        480 
QCFLEESRQL MRLPPRQSTG ESDLMVPFQL AATKTIAEED KYFSRVSLRM PSFGVTVRSS 

       490        500        510        520        530        540 
QADMVRSKVS LTGKANLTSD TSVDGWDAIA LEYSVDWPMQ LFFTQEVLSK YLKVFQYLIR 

       550        560        570        580        590        600 
LKRTQMELEK SWASVMHQDH IESAQHRKDG LNGSTSQQRR QGIRPMWRVR EHMAFLIRNL 

       610        620        630        640        650        660 
QFYIQVDVIE SQWKVLQTHI HDSQDFTELV GFHQEYLSAL ISQSFLDIGS VSRILDSIMK 

       670        680        690        700        710        720 
LCLQFCWNIE NQESNPNTSE LENIAEEFNK KSNSLYTILR SSKLAGSQRA PFLRRFLLRL 

       730        740 
NFNSFYEATA RGVLNVVRQR PALPL 

References and documentation are available.

Number of amino acids: 745

Molecular weight: 85907.22

Theoretical pI: 6.43

Amino acid composition: 
Ala (A)  41	  5.5%
Arg (R)  55	  7.4%
Asn (N)  23	  3.1%
Asp (D)  42	  5.6%
Cys (C)   8	  1.1%
Gln (Q)  42	  5.6%
Glu (E)  56	  7.5%
Gly (G)  34	  4.6%
His (H)  21	  2.8%
Ile (I)  39	  5.2%
Leu (L)  94	 12.6%
Lys (K)  37	  5.0%
Met (M)  18	  2.4%
Phe (F)  41	  5.5%
Pro (P)  26	  3.5%
Ser (S)  67	  9.0%
Thr (T)  32	  4.3%
Trp (W)  11	  1.5%
Tyr (Y)  17	  2.3%
Val (V)  41	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      3838
Hydrogen    H	      6043
Nitrogen    N	      1065
Oxygen      O	      1123
Sulfur      S	        26

Formula: C3838H6043N1065O1123S26
Total number of atoms: 12095

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    86330
Abs 0.1% (=1 g/l)   1.005, assuming all pairs of Cys residues form cystines


Ext. coefficient    85830
Abs 0.1% (=1 g/l)   0.999, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.80
This classifies the protein as unstable.



Aliphatic index: 91.09

Grand average of hydropathicity (GRAVY): -0.329