ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR GFHGDSVVQQ 

        70         80         90        100        110        120 
IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI SGMHGLGAEI AKNLILAGVK 

       130        140        150        160        170        180 
SVTLHDERVV ELWDLSSNFV FSEDDVGKNR ADASVQKLQD LNNAVVVSSL TKSLNKEDLS 

       190        200        210        220        230        240 
GFQVVVFSDI SMERAIEFDD YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE 

       250        260        270        280        290        300 
PHTGIIASIS NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF 

       310        320        330        340        350        360 
TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF DRPPLLHLAF 

       370        380        390        400        410        420 
QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK VENVDQKLLR HFSFGAKAVL 

       430        440        450        460        470        480 
NPMAAMFGGI VGQEVVKACS GKFHPLFQFF YFDSVESLPS EPVDSSDFAP RNSRYDAQIS 

       490        500        510        520        530        540 
VFGAKFQKKL EDAKVFTVGS GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ 

       550        560        570        580        590        600 
FLFRDWNIGQ AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD 

       610        620        630        640        650        660 
NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE KQAPMCTVHS 

       670        680        690        700        710        720 
FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS MMSAGDAQAR DTLERIVECL 

       730        740        750        760        770        780 
EKEKCETFQD CLTWARLRFE DYFVNRVKQL IYTFPEDAAT STGAPFWSAP KRFPRPLQYS 

       790        800        810        820        830        840 
SSDPSLLNFI TATAILRAET FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK 

       850        860        870        880        890        900 
ATTLTTASVD DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKGDDTNYHM DVIAGLANMR 

       910        920        930        940        950        960 
ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV EAYRNTFANL 

       970        980        990       1000       1010       1020 
ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV LQWLEDKGLS AYSISCGSCL 

      1030       1040       1050       1060       1070       1080 
LFNSMFTRHK ERMDKKVVDL ARDVAKVELP PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR 

References and documentation are available.

Number of amino acids: 1080

Molecular weight: 120192.94

Theoretical pI: 5.13

Amino acid composition: 
Ala (A)  93	  8.6%
Arg (R)  54	  5.0%
Asn (N)  50	  4.6%
Asp (D)  86	  8.0%
Cys (C)  18	  1.7%
Gln (Q)  34	  3.1%
Glu (E)  70	  6.5%
Gly (G)  58	  5.4%
His (H)  22	  2.0%
Ile (I)  51	  4.7%
Leu (L)  94	  8.7%
Lys (K)  64	  5.9%
Met (M)  20	  1.9%
Phe (F)  64	  5.9%
Pro (P)  49	  4.5%
Ser (S)  84	  7.8%
Thr (T)  52	  4.8%
Trp (W)  11	  1.0%
Tyr (Y)  22	  2.0%
Val (V)  84	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 156
Total number of positively charged residues (Arg + Lys): 118

Atomic composition:

Carbon      C	      5343
Hydrogen    H	      8335
Nitrogen    N	      1445
Oxygen      O	      1635
Sulfur      S	        38

Formula: C5343H8335N1445O1635S38
Total number of atoms: 16796

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94405
Abs 0.1% (=1 g/l)   0.785, assuming all pairs of Cys residues form cystines


Ext. coefficient    93280
Abs 0.1% (=1 g/l)   0.776, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.19
This classifies the protein as stable.



Aliphatic index: 83.53

Grand average of hydropathicity (GRAVY): -0.257