ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLHLALAALA LVAQFNVPRY TEAPVYYVDV VNLPVASPRA GSPTVGGAPP ELPPPASQAM 

        70         80         90        100        110        120 
ATPRLTPHTP AAVGKKSPTA ADTGKEFEDR LSALQRNVDE RRQEAALDAL RRKVNGSGRA 

       130        140        150        160        170        180 
EPAGMPSATG TQAGSDYAAY IQSRLRDAFR YTIAHQAKTP EVVIRLTIDP RGRVTRQRIE 

       190        200        210        220 
RSTGDRIFEE SVMKAIAMAE KTFAPPPSGG EFETGFIFRP EGVGKR 

References and documentation are available.

Number of amino acids: 226

Molecular weight: 24347.68

Theoretical pI: 9.73

Amino acid composition: 
Ala (A)  33	 14.6%
Arg (R)  22	  9.7%
Asn (N)   4	  1.8%
Asp (D)   9	  4.0%
Cys (C)   0	  0.0%
Gln (Q)   8	  3.5%
Glu (E)  15	  6.6%
Gly (G)  17	  7.5%
His (H)   3	  1.3%
Ile (I)   8	  3.5%
Leu (L)  14	  6.2%
Lys (K)   8	  3.5%
Met (M)   5	  2.2%
Phe (F)   8	  3.5%
Pro (P)  22	  9.7%
Ser (S)  12	  5.3%
Thr (T)  16	  7.1%
Trp (W)   0	  0.0%
Tyr (Y)   6	  2.7%
Val (V)  16	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1071
Hydrogen    H	      1720
Nitrogen    N	       318
Oxygen      O	       321
Sulfur      S	         5

Formula: C1071H1720N318O321S5
Total number of atoms: 3435

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.367

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.57
This classifies the protein as unstable.



Aliphatic index: 73.10

Grand average of hydropathicity (GRAVY): -0.392