ProtParam
User-provided sequence:
10 20 30 40 50 60
MLHVGEESFQ MSTLLERTKS VQELKKRAAG KTSANPAEVA KAKKVLRRLY SWDEIPEWQR
70 80 90 100 110 120
DNDFILHGYV KETSSFIETF KSLFYLHNES VNIYSHLIPA LGFFTVLLLD KSTIKVFATT
130 140 150 160 170 180
TWLDHMVIDL FYSGAFACLI LSSSFHCLKS HSLRIATLGN KLDYLGICIL IVTSMVSILY
190 200 210 220 230 240
YGYFEKFSLF CLFALITVSF GIACSIVSLK DKFRKREWRP YRAGLFVCFG LSSIIPIFSG
250 260 270 280 290 300
LYCYSFSEIW TQIQLFWVLL GGVLYIIGAV LYGMRFPEKI CPGKFDIWGH SHQLFHFLVV
310 320
IAALCHLRGL LNSYELVHIK MENGIVS
References and
documentation are available.
Number of amino acids: 327
Molecular weight: 37437.08
Theoretical pI: 8.85
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1753
Hydrogen H 2683
Nitrogen N 427
Oxygen O 451
Sulfur S 15
Formula: C
1753H
2683N
427O
451S
15
Total number of atoms: 5329
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 61350
Abs 0.1% (=1 g/l) 1.639, assuming all pairs of Cys residues form cystines
Ext. coefficient 60850
Abs 0.1% (=1 g/l) 1.625, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.28
This classifies the protein as stable.
Aliphatic index: 109.69
Grand average of hydropathicity (GRAVY): 0.415