ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS ATYVKPDIIL 

        70         80         90        100        110        120 
FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF QCYTIIGYPE DDDEQKLYNS 

       130        140        150        160        170        180 
ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL 

       190        200        210        220        230        240 
KASIGICMDL SPYKFMAPFN HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL 

       250        260        270        280        290        300 
EAAKNKIAFA LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY 

       310        320        330        340        350        360 
WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI DLLDSEEVIK 

       370        380        390        400        410        420 
DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI FAGSSGIYKF NGKKPKGSQD 

       430        440        450 
DDESSLDSLN ESVELLGNLG KGLEGAILRE VQFEVFR 

References and documentation are available.

Number of amino acids: 457

Molecular weight: 51896.95

Theoretical pI: 5.09

Amino acid composition: 
Ala (A)  20	  4.4%
Arg (R)  12	  2.6%
Asn (N)  24	  5.3%
Asp (D)  34	  7.4%
Cys (C)   7	  1.5%
Gln (Q)  18	  3.9%
Glu (E)  31	  6.8%
Gly (G)  27	  5.9%
His (H)   6	  1.3%
Ile (I)  30	  6.6%
Leu (L)  46	 10.1%
Lys (K)  39	  8.5%
Met (M)  10	  2.2%
Phe (F)  27	  5.9%
Pro (P)  20	  4.4%
Ser (S)  38	  8.3%
Thr (T)  25	  5.5%
Trp (W)   6	  1.3%
Tyr (Y)  17	  3.7%
Val (V)  20	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2336
Hydrogen    H	      3614
Nitrogen    N	       592
Oxygen      O	       710
Sulfur      S	        17

Formula: C2336H3614N592O710S17
Total number of atoms: 7269

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58705
Abs 0.1% (=1 g/l)   1.131, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   1.124, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.71
This classifies the protein as stable.



Aliphatic index: 81.93

Grand average of hydropathicity (GRAVY): -0.385