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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLIEEIEFYN VNGKKTTTVV PENTKIKKRV LNDRRTLYVG NLPKNCRKQD LRDLFEPNYG

70 80 90 100 110 120
KITINMLKKK PLKKPLKRFA FIEFQEGVNL KKVKEKMNGK IFMNEKIVIE NILTKEEKSF

130 140 150 160 170 180
EKNQKSNKKT APDLKPLSTN TLYVKNIPMK STNEDLAKIF GVDPKNINFV RRELVDLRTN

190 200 210 220 230 240
KVFFSDEFHT GEAFIKFDNL GTGDSIQKKC REFKGRKASN GRVLLVKIAS AKKNEQKQEG


GDNTKIKQN


References and documentation are available.

Number of amino acids: 249 Molecular weight: 28861.65 Theoretical pI: 9.91
Amino acid composition: 
Ala (A) 7 2.8% Arg (R) 12 4.8% Asn (N) 25 10.0% Asp (D) 11 4.4% Cys (C) 2 0.8% Gln (Q) 7 2.8% Glu (E) 20 8.0% Gly (G) 13 5.2% His (H) 1 0.4% Ile (I) 17 6.8% Leu (L) 20 8.0% Lys (K) 43 17.3% Met (M) 5 2.0% Phe (F) 15 6.0% Pro (P) 9 3.6% Ser (S) 8 3.2% Thr (T) 15 6.0% Trp (W) 0 0.0% Tyr (Y) 4 1.6% Val (V) 15 6.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 31 Total number of positively charged residues (Arg + Lys): 55 Atomic composition: Carbon C 1290 Hydrogen H 2120 Nitrogen N 362 Oxygen O 371 Sulfur S 7 Formula: C1290H2120N362O371S7 Total number of atoms: 4150 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 6085 Abs 0.1% (=1 g/l) 0.211, assuming all pairs of Cys residues form cystines Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.207, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.13 This classifies the protein as stable. Aliphatic index: 78.23 Grand average of hydropathicity (GRAVY): -0.813