ProtParam
User-provided sequence:
10 20 30 40 50 60
MLIEEIEFYN VNGKKTTTVV PENTKIKKRV LNDRRTLYVG NLPKNCRKQD LRDLFEPNYG
70 80 90 100 110 120
KITINMLKKK PLKKPLKRFA FIEFQEGVNL KKVKEKMNGK IFMNEKIVIE NILTKEEKSF
130 140 150 160 170 180
EKNQKSNKKT APDLKPLSTN TLYVKNIPMK STNEDLAKIF GVDPKNINFV RRELVDLRTN
190 200 210 220 230 240
KVFFSDEFHT GEAFIKFDNL GTGDSIQKKC REFKGRKASN GRVLLVKIAS AKKNEQKQEG
GDNTKIKQN
References and
documentation are available.
Number of amino acids: 249
Molecular weight: 28861.65
Theoretical pI: 9.91
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 1290
Hydrogen H 2120
Nitrogen N 362
Oxygen O 371
Sulfur S 7
Formula: C
1290H
2120N
362O
371S
7
Total number of atoms: 4150
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.211, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.207, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.13
This classifies the protein as stable.
Aliphatic index: 78.23
Grand average of hydropathicity (GRAVY): -0.813