ProtParam
User-provided sequence:
10 20 30 40 50 60
MLINHLSKIR TVRHFSNIKP VLSKEVSRRV IVAPASHFKT SSPNVKSNIP IHEYKQLPED
70 80 90 100 110 120
SNYIEKHYKE LQVFLNEFLI KKLNKTYADF EGDPDELVFQ LEKFIELEVT PRYTNHSAPD
130 140 150 160 170
GCEERFKSIG DRIVVDRYLD FVKDVRLTLL LNGGHSFIFD VMLQAKEVFD KMQKE
References and
documentation are available.
Number of amino acids: 175
Molecular weight: 20512.60
Theoretical pI: 7.07
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 932
Hydrogen H 1463
Nitrogen N 247
Oxygen O 266
Sulfur S 4
Formula: C
932H
1463N
247O
266S
4
Total number of atoms: 2912
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.436, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.436, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.62
This classifies the protein as stable.
Aliphatic index: 92.34
Grand average of hydropathicity (GRAVY): -0.418