ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLIRLKKRKI LQVIVSAVVL ILFFCSVHND VSSSWLYGKK LRLPVLTRSN LKNNFYTTLV 

        70         80         90        100        110        120 
QAIVENKPAD SSPDLSKLHG AEGCSFANNV AAHDSGHDSD LSYESLSKCY NLNKTVQESL 

       130        140        150        160        170        180 
REVHSKFTDT LSGKLNFSIP QREALFSGSE GIVTIGGGKY SVLAYTMIKK LRDTGTTLPI 

       190        200        210        220        230        240 
EVIIPPQDEG EDDFCKNWLP KFNGKCIYFS DIVPSKPLSD LKLTHFQLKV FGLIISSFKR 

       250        260        270        280        290        300 
IIFLDADNYA VKNLDLAFNT TSFNDTGLIL WPDFWRRVTP PAFYNIIGSS INIGKRVRFV 

       310        320        330        340        350        360 
SDDISPVSRY DPFVSNSNDY TPKERQEHFL KHVPLHDLDG TMPDLSSESG QMVIDKIRHF 

       370        380        390        400        410        420 
NTLLLALYYN VYGPTWYYKM ISQGTAGEGD KDTFFAAAHA LNMPYYQVRT NFEFDGFFYQ 

       430        440        450        460        470        480 
KDDYKGLALL QHDFEQDYKQ YQKAQQKVKA NIEEFSKLDP DYTLDNGFLK TLMVNDDGSD 

       490        500        510        520        530        540 
LDIMFIHASF YKADPWTLYH ENRFIGPNGE QVRGFRKPHR YGMDFELFLF NDMRGSFCTT 

       550        560        570        580 
PKSQVIKFKY FTDKVNTPEW DAMCEYLTNH VNYLESTHKE AMGEKN 

References and documentation are available.

Number of amino acids: 586

Molecular weight: 67252.39

Theoretical pI: 6.39

Amino acid composition: 
Ala (A)  26	  4.4%
Arg (R)  21	  3.6%
Asn (N)  34	  5.8%
Asp (D)  45	  7.7%
Cys (C)   7	  1.2%
Gln (Q)  19	  3.2%
Glu (E)  26	  4.4%
Gly (G)  33	  5.6%
His (H)  17	  2.9%
Ile (I)  32	  5.5%
Leu (L)  56	  9.6%
Lys (K)  45	  7.7%
Met (M)  12	  2.0%
Phe (F)  41	  7.0%
Pro (P)  26	  4.4%
Ser (S)  45	  7.7%
Thr (T)  32	  5.5%
Trp (W)   7	  1.2%
Tyr (Y)  29	  4.9%
Val (V)  33	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      3057
Hydrogen    H	      4644
Nitrogen    N	       788
Oxygen      O	       888
Sulfur      S	        19

Formula: C3057H4644N788O888S19
Total number of atoms: 9396

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82085
Abs 0.1% (=1 g/l)   1.221, assuming all pairs of Cys residues form cystines


Ext. coefficient    81710
Abs 0.1% (=1 g/l)   1.215, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.85
This classifies the protein as stable.



Aliphatic index: 79.33

Grand average of hydropathicity (GRAVY): -0.373