ProtParam
User-provided sequence:
10 20 30 40 50 60
MLITETFHDV QTSYGTTLRI YVYSPKIAGY PQAKFPGVIL YSEIYQVTGP VRRFGQRIAS
70 80 90 100 110 120
EGYVVVAPAI YHNFMGPEAL PYDVQGTDIG NEYKIKKPLE SYDEDNKLCC DLLFQLPQFD
130 140 150 160 170 180
GKRIGSTGMC LGGHLAFRAL LDKRVTCATC FFPTDIHSRT LGLGQNDNSL ERVSKELGNN
190 200 210 220 230 240
QEMVLIFGTA DTHVDPQGRD LIRKTLRDHG VKFTFLEILA AQHAFIRDEF SKGRFDSAIT
250 260 270
QSCLGFLFEQ FNRKLRIDLG EFVDDNTPLE HVC
References and
documentation are available.
Number of amino acids: 273
Molecular weight: 30837.15
Theoretical pI: 5.91
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1385
Hydrogen H 2142
Nitrogen N 372
Oxygen O 405
Sulfur S 11
Formula: C
1385H
2142N
372O
405S
11
Total number of atoms: 4315
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16765
Abs 0.1% (=1 g/l) 0.544, assuming all pairs of Cys residues form cystines
Ext. coefficient 16390
Abs 0.1% (=1 g/l) 0.532, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.03
This classifies the protein as stable.
Aliphatic index: 84.98
Grand average of hydropathicity (GRAVY): -0.218