ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKEPSVRLR EAIIEGNLLI VKRLLRRNPD LLTNIDSENG WSSLHYASYH GRYLICVYLI 

        70         80         90        100        110        120 
QLGHDKHELI KTFKGNTCVH LALMKGHEQT LHLLLQQFPR FINHRGENGR APIHIACMND 

       130        140        150        160        170        180 
YYQCLSLLIG VGADLWVMDT NGDTPLHVCL EYGSISCMKM LLNEGEVSLD DNVRDKGNWK 

       190        200        210        220        230        240 
PIDVAQTFEV GNIYSKVLKE VKKKGPPLGA GKKPSSFRTP ILNAKATFED GPSPVLSMNS 

       250        260        270        280        290        300 
PYSLYSNNSP LPVLPRRIST HTTSGNGGNR RSSITNPVFN PRKPTLSTDS FSSSSNSSSR 

       310        320        330        340        350        360 
LRVNSINVKT PVGVSPKKEL VSESVRHSAT PTSPHNNIAL INRYLLPNKS NDNVRGDSQT 

       370        380        390        400        410        420 
ATINDDGGGG NGGDATIGMG LRKDPDDENE NKYKIKVNNG EPRRRVSLLN IPISKLRNSN 


NTRAED 

References and documentation are available.

Number of amino acids: 426

Molecular weight: 47139.48

Theoretical pI: 9.39

Amino acid composition: 
Ala (A)  15	  3.5%
Arg (R)  27	  6.3%
Asn (N)  40	  9.4%
Asp (D)  22	  5.2%
Cys (C)   6	  1.4%
Gln (Q)   7	  1.6%
Glu (E)  19	  4.5%
Gly (G)  33	  7.7%
His (H)  13	  3.1%
Ile (I)  25	  5.9%
Leu (L)  45	 10.6%
Lys (K)  27	  6.3%
Met (M)   8	  1.9%
Phe (F)   8	  1.9%
Pro (P)  27	  6.3%
Ser (S)  42	  9.9%
Thr (T)  22	  5.2%
Trp (W)   3	  0.7%
Tyr (Y)  12	  2.8%
Val (V)  25	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2056
Hydrogen    H	      3314
Nitrogen    N	       610
Oxygen      O	       632
Sulfur      S	        14

Formula: C2056H3314N610O632S14
Total number of atoms: 6626

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34755
Abs 0.1% (=1 g/l)   0.737, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.729, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.75
This classifies the protein as unstable.



Aliphatic index: 84.62

Grand average of hydropathicity (GRAVY): -0.545