ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKFRTVHGG LRLLGIRRTS TAPAASPNVR RLEYKPIKKV MVANRGEIAI RVFRACTELG 

        70         80         90        100        110        120 
IRTVAIYSEQ DTGQMHRQKA DEAYLIGRGL APVQAYLHIP DIIKVAKENN VDAVHPGYGF 

       130        140        150        160        170        180 
LSERADFAQA CQDAGVRFIG PSPEVVRKMG DKVEARAIAI AAGVPVVPGT DAPITSLHEA 

       190        200        210        220        230        240 
HEFSNTYGFP IIFKAAYGGG GRGMRVVHSY EELEENYTRA YSEALAAFGN GALFVEKFIE 

       250        260        270        280        290        300 
KPRHIEVQIL GDQYGNILHL YERDCSIQRR HQKVVEIAPA AHLDPQLRTR LTSDSVKLAK 

       310        320        330        340        350        360 
QVGYENAGTV EFLVDRHGKH YFIEVNSRLQ VEHTVTEEIT DVDLVHAQIH VAEGRSLPDL 

       370        380        390        400        410        420 
GLRQENIRIN GCAIQCRVTT EDPARSFQPD TGRIEVFRSG EGMGIRLDNA SAFQGAVISP 

       430        440        450        460        470        480 
HYDSLLVKVI AHGKDHPTAA TKMSRALAEF RVRGVKTNIA FLQNVLNNQQ FLAGTVDTQF 

       490        500        510        520        530        540 
IDENPELFQL RPAQNRAQKL LHYLGHVMVN GPTTPIPVKA SPSPTDPVVP AVPIGPPPAG 

       550        560        570        580        590        600 
FRDILLREGP EGFARAVRNH PGLLLMDTTF RDAHQSLLAT RVRTHDLKKI APYVAHNFSK 

       610        620        630        640        650        660 
LFSMENWGGA TFDVAMRFLY ECPWRRLQEL RELIPNIPFQ MLLRGANAVG YTNYPDNVVF 

       670        680        690        700        710        720 
KFCEVAKENG MDVFRVFDSL NYLPNMLLGM EAAGSAGGVV EAAISYTGDV ADPSRTKYSL 

       730        740        750        760        770        780 
QYYMGLAEEL VRAGTHILCI KDMAGLLKPT ACTMLVSSLR DRFPDLPLHI HTHDTSGAGV 

       790        800        810        820        830        840 
AAMLACAQAG ADVVDVAADS MSGMTSQPSM GALVACTRGT PLDTEVPMER VFDYSEYWEG 

       850        860        870        880        890        900 
ARGLYAAFDC TATMKSGNSD VYENEIPGGQ YTNLHFQAHS MGLGSKFKEV KKAYVEANQM 

       910        920        930        940        950        960 
LGDLIKVTPS SKIVGDLAQF MVQNGLSRAE AEAQAEELSF PRSVVEFLQG YIGVPHGGFP 

       970        980        990       1000       1010       1020 
EPFRSKVLKD LPRVEGRPGA SLPPLDLQAL EKELVDRHGE EVTPEDVLSA AMYPDVFAHF 

      1030       1040       1050       1060       1070       1080 
KDFTATFGPL DSLNTRLFLQ GPKIAEEFEV ELERGKTLHI KALAVSDLNR AGQRQVFFEL 

      1090       1100       1110       1120       1130       1140 
NGQLRSILVK DTQAMKEMHF HPKALKDVKG QIGAPMPGKV IDIKVVAGAK VAKGQPLCVL 

      1150       1160       1170 
SAMKMETVVT SPMEGTVRKV HVTKDMTLEG DDLILEIE 

References and documentation are available.

Number of amino acids: 1178

Molecular weight: 129633.55

Theoretical pI: 6.37

Amino acid composition: 
Ala (A) 116	  9.8%
Arg (R)  72	  6.1%
Asn (N)  38	  3.2%
Asp (D)  61	  5.2%
Cys (C)  13	  1.1%
Gln (Q)  46	  3.9%
Glu (E)  79	  6.7%
Gly (G)  94	  8.0%
His (H)  38	  3.2%
Ile (I)  56	  4.8%
Leu (L) 105	  8.9%
Lys (K)  56	  4.8%
Met (M)  35	  3.0%
Phe (F)  52	  4.4%
Pro (P)  67	  5.7%
Ser (S)  55	  4.7%
Thr (T)  59	  5.0%
Trp (W)   3	  0.3%
Tyr (Y)  33	  2.8%
Val (V) 100	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 140
Total number of positively charged residues (Arg + Lys): 128

Atomic composition:

Carbon      C	      5767
Hydrogen    H	      9123
Nitrogen    N	      1613
Oxygen      O	      1690
Sulfur      S	        48

Formula: C5767H9123N1613O1690S48
Total number of atoms: 18241

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66420
Abs 0.1% (=1 g/l)   0.512, assuming all pairs of Cys residues form cystines


Ext. coefficient    65670
Abs 0.1% (=1 g/l)   0.507, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.02
This classifies the protein as stable.



Aliphatic index: 87.77

Grand average of hydropathicity (GRAVY): -0.169