ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKHYIHQCV KASSCKHSLS VKQRYFHCSA LNGVASMFRM PALSPTMEEG NITKWHFKEG 

        70         80         90        100        110        120 
DSFKSGDILL EVETDKATMD VEVQDNGILA KVLIEKGSNI PVGKNIAIVA DAEDNLKDLE 

       130        140        150        160        170        180 
LPKDEASSEE QSFSSSKEEV KPIVQDRETK SNVEHKSTSQ ANDAVNKSFL PSVSYLIHQY 

       190        200        210        220        230        240 
KIENPWSIPA TGPHGRLLKG DVLAHVGKID KGVPSSLQKF VRNLEVLDFS GVEPKKPSYT 

       250        260        270        280        290        300 
EDSVPVRKST MIETLKTVPM KDEYITFERS ISLEKLKSLL AKRKEKDAFG IDEVVSAMIG 

       310        320        330        340        350        360 
DALSISELSK VEPNSIESAY DLLLGAPIVK LKSSVSNFQP KLFPKNNNTK DLHKYIRDSM 

       370        380        390        400        410        420 
IGLPSNLSDY VKLNFSSVTK SSSNNVEFLD DVYDMLLGSN SDSIDSRINR HEEPSTTEDL 

       430        440        450 
TLTLSIKDNI SKSRAIKFVD CLKSNFENPE FVLSRW 

References and documentation are available.

Number of amino acids: 456

Molecular weight: 50883.83

Theoretical pI: 5.96

Amino acid composition: 
Ala (A)  21	  4.6%
Arg (R)  13	  2.9%
Asn (N)  25	  5.5%
Asp (D)  31	  6.8%
Cys (C)   4	  0.9%
Gln (Q)   9	  2.0%
Glu (E)  35	  7.7%
Gly (G)  18	  3.9%
His (H)  11	  2.4%
Ile (I)  28	  6.1%
Leu (L)  43	  9.4%
Lys (K)  46	 10.1%
Met (M)  10	  2.2%
Phe (F)  17	  3.7%
Pro (P)  21	  4.6%
Ser (S)  59	 12.9%
Thr (T)  18	  3.9%
Trp (W)   3	  0.7%
Tyr (Y)  10	  2.2%
Val (V)  34	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2251
Hydrogen    H	      3606
Nitrogen    N	       600
Oxygen      O	       710
Sulfur      S	        14

Formula: C2251H3606N600O710S14
Total number of atoms: 7181

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31650
Abs 0.1% (=1 g/l)   0.622, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.617, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.87
This classifies the protein as unstable.



Aliphatic index: 86.95

Grand average of hydropathicity (GRAVY): -0.423