ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKIKALFSK KKPDQADLSQ ESKKPFKGKT RSSGTNNKDV SQITSSPKKS FQDKNIVQYP 

        70         80         90        100        110        120 
SVVADDHHMK SLTDELVTTI DSDSSPSDNI TTENVETVTS VPAIDVHESS EGQLSSDPLI 

       130        140        150        160        170        180 
SDESLSEQSE IISDIQDDST DDDNMEDEIP EKSFLEQKEL IGYKLINKIG EGAFSKVFRA 

       190        200        210        220        230        240 
IPAKNSSNEF LTKNYKAVAI KVIKKADLSS INGDHRKKDK GKDSTKTSSR DQVLKEVALH 

       250        260        270        280        290        300 
KTVSAGCSQI VAFIDFQETD SYYYIIQELL TGGEIFGEIV RLTYFSEDLS RHVIKQLALA 

       310        320        330        340        350        360 
VKHMHSLGVV HRDIKPENLL FEPIEFTRSI KPKLRKSDDP QTKADEGIFT PGVGGGGIGI 

       370        380        390        400        410        420 
VKLADFGLSK QIFSKNTKTP CGTVGYTAPE VVKDEHYSMK VDMWGIGCVL YTMLCGFPPF 

       430        440        450        460        470        480 
YDEKIDTLTE KISRGEYTFL KPWWDEISAG AKNAVAKLLE LEPSKRYDID QFLDDPWLNT 

       490        500        510        520        530        540 
FDCLPKEGES SQKKAGTSER RHPHKKQFQL FQRDSSLLFS PAAVAMRDAF DIGNAVKRTE 

       550        560        570        580        590        600 
EDRMGTRGGL GSLAEDEELE DSYSGAQGDE QLEQNMFQLT LDTSTILQRR KKVQENDVGP 

       610 
TIPISATIRE 

References and documentation are available.

Number of amino acids: 610

Molecular weight: 68061.58

Theoretical pI: 5.28

Amino acid composition: 
Ala (A)  31	  5.1%
Arg (R)  21	  3.4%
Asn (N)  18	  3.0%
Asp (D)  52	  8.5%
Cys (C)   5	  0.8%
Gln (Q)  27	  4.4%
Glu (E)  47	  7.7%
Gly (G)  38	  6.2%
His (H)  12	  2.0%
Ile (I)  42	  6.9%
Leu (L)  47	  7.7%
Lys (K)  59	  9.7%
Met (M)  10	  1.6%
Phe (F)  26	  4.3%
Pro (P)  26	  4.3%
Ser (S)  60	  9.8%
Thr (T)  38	  6.2%
Trp (W)   4	  0.7%
Tyr (Y)  14	  2.3%
Val (V)  33	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3001
Hydrogen    H	      4767
Nitrogen    N	       805
Oxygen      O	       966
Sulfur      S	        15

Formula: C3001H4767N805O966S15
Total number of atoms: 9554

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43110
Abs 0.1% (=1 g/l)   0.633, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.630, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.77
This classifies the protein as unstable.



Aliphatic index: 77.67

Grand average of hydropathicity (GRAVY): -0.580