ProtParam
User-provided sequence:
10 20 30 40 50 60
MLKIKRVPTV VSNYQKDDGA EDPVGCGRNC LGACCLNGAR LPLYACKNLV KSGEKLVISH
70 80 90 100 110 120
EAIEPPVAFL ESLVLGEWED RFQRGLFRYD VTACETKVIP GKYGFVAQLN EGRHLKKRPT
130 140 150 160 170 180
EFRVDKVLQS FDGSKFNFTK VGQEELLFQF EAGEDAQVQF FPCMPIDPEN SPSVVAINVS
190 200 210 220 230 240
PIEYGHVLLI PRVLDCLPQR IDHKSLLLAV HMAAEAANPY FRLGYNSLGA FATINHLHFQ
250 260 270 280 290 300
AYYLAMPFPL EKAPTKKITT TVSGVKISEL LSYPVRSLLF EGGSSMQELS DTVSDCCVCL
310 320 330 340 350 360
QNNNIPFNIL ISDCGRQIFL MPQCYAEKQA LGEVSPEVLE TQVNPAVWEI SGHMVLKRKE
370 380 390 400 410 420
DYEGASEDNA WRLLAEASLS EERFKEVTAL AFEAIGCSNQ EEDLEGTIVH QQNSSGNVNQ
430 440
KSNRTHGGPI TNGTAAECLV LQ
References and
documentation are available.
Number of amino acids: 442
Molecular weight: 48969.90
Theoretical pI: 5.29
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 2176
Hydrogen H 3414
Nitrogen N 586
Oxygen O 655
Sulfur S 22
Formula: C
2176H
3414N
586O
655S
22
Total number of atoms: 6853
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35255
Abs 0.1% (=1 g/l) 0.720, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 0.702, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.14
This classifies the protein as unstable.
Aliphatic index: 88.67
Grand average of hydropathicity (GRAVY): -0.163