ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKLSRSANL RLVQLPAARL SGNGAKLLTQ RGFFTVTRLW QSNGKKPLSR VPVGGTPIKD 

        70         80         90        100        110        120 
NGKVREGSIE FSTGKAIALF LAVGGALSYF FNREKRRLET QKEAEANRGY GKPSLGGPFH 

       130        140        150        160        170        180 
LEDMYGNEFT EKNLLGKFSI IYFGFSNCPD ICPDELDKLG LWLNTLSSKY GITLQPLFIT 

       190        200        210        220        230        240 
CDPARDSPAV LKEYLSDFHP SILGLTGTFD EVKNACKKYR VYFSTPPNVK PGQDYLVDHS 

       250        260        270        280        290 
IFFYLMDPEG QFVDALGRNY DEKTGVDKIV EHVKSYVPAE QRAKQKEAWY SFLFK 

References and documentation are available.

Number of amino acids: 295

Molecular weight: 33166.00

Theoretical pI: 9.15

Amino acid composition: 
Ala (A)  17	  5.8%
Arg (R)  15	  5.1%
Asn (N)  13	  4.4%
Asp (D)  15	  5.1%
Cys (C)   4	  1.4%
Gln (Q)   9	  3.1%
Glu (E)  17	  5.8%
Gly (G)  26	  8.8%
His (H)   4	  1.4%
Ile (I)  11	  3.7%
Leu (L)  33	 11.2%
Lys (K)  25	  8.5%
Met (M)   3	  1.0%
Phe (F)  20	  6.8%
Pro (P)  17	  5.8%
Ser (S)  20	  6.8%
Thr (T)  14	  4.7%
Trp (W)   3	  1.0%
Tyr (Y)  13	  4.4%
Val (V)  16	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1511
Hydrogen    H	      2336
Nitrogen    N	       398
Oxygen      O	       429
Sulfur      S	         7

Formula: C1511H2336N398O429S7
Total number of atoms: 4681

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36120
Abs 0.1% (=1 g/l)   1.089, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   1.082, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.38
This classifies the protein as stable.



Aliphatic index: 79.66

Grand average of hydropathicity (GRAVY): -0.357