ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKNFKLSKR DSNGSKGRIT SADISTPSHD NGSVIKHIKT VPVRYLSSSS TPVKSQRDSS 

        70         80         90        100        110        120 
PKNRHNSKDI TSPEKVIKAK YSYQAQTSKE LSFMEGEFFY VSGDEKDWYK ASNPSTGKEG 

       130        140        150        160        170        180 
VVPKTYFEVF DRTKPSSVNG SNSSSRKVTN DSLNMGSLYA IVLYDFKAEK ADELTTYVGE 

       190        200        210        220        230        240 
NLFICAHHNC EWFIAKPIGR LGGPGLVPVG FVSIIDIATG YATGNDVIED IKSVNLPTVQ 

       250        260        270        280        290        300 
EWKSNIARYK ASNISLGSVE QQQQQSITKP QNKSAKLVDG ELLVKASVES FGLEDEKYWF 

       310        320        330        340        350        360 
LVCCELSNGK TRQLKRYYQD FYDLQVQLLD AFPAEAGKLR DAGGQWSKRI MPYIPGPVPY 

       370        380        390        400        410        420 
VTNSITKKRK EDLNIYVADL VNLPDYISRS EMVHSLFVVL NNGFDREFER DENQNNIKTL 

       430        440        450        460        470        480 
QENDTATFAT ASQTSNFAST NQDNTLTGED LKLNKKLSDL SLSGSKQAPA QSTSGLKTTK 

       490        500        510        520        530        540 
IKFYYKDDIF ALMLKGDTTY KELRSKIAPR IDTDNFKLQT KLFDGSGEEI KTDSQVSNII 

       550 
QAKLKISVHD I 

References and documentation are available.

Number of amino acids: 551

Molecular weight: 61605.37

Theoretical pI: 8.71

Amino acid composition: 
Ala (A)  29	  5.3%
Arg (R)  19	  3.4%
Asn (N)  33	  6.0%
Asp (D)  37	  6.7%
Cys (C)   4	  0.7%
Gln (Q)  24	  4.4%
Glu (E)  30	  5.4%
Gly (G)  32	  5.8%
His (H)   7	  1.3%
Ile (I)  33	  6.0%
Leu (L)  43	  7.8%
Lys (K)  54	  9.8%
Met (M)   6	  1.1%
Phe (F)  23	  4.2%
Pro (P)  21	  3.8%
Ser (S)  60	 10.9%
Thr (T)  35	  6.4%
Trp (W)   5	  0.9%
Tyr (Y)  22	  4.0%
Val (V)  34	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      2734
Hydrogen    H	      4316
Nitrogen    N	       738
Oxygen      O	       860
Sulfur      S	        10

Formula: C2734H4316N738O860S10
Total number of atoms: 8658

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60530
Abs 0.1% (=1 g/l)   0.983, assuming all pairs of Cys residues form cystines


Ext. coefficient    60280
Abs 0.1% (=1 g/l)   0.978, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.83
This classifies the protein as stable.



Aliphatic index: 76.95

Grand average of hydropathicity (GRAVY): -0.566