ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKQNNGLHL ARRNLWVVCS GLRAKDCDSG GKGGKGKENG KFRTEKDTFG ELKVPADKLY 

        70         80         90        100        110        120 
GAQTMRSKLN FPIGDIGERM PMPVIQAMGI LKKACAEVNK DYGLDGKVSD AVSCACDDVI 

       130        140        150        160        170        180 
SGKLYKQGHF PLVIWQTGSG TQTNMNTNEV ISNAAIKMMG GELGSKKPVH PNDHVNKSQS 

       190        200        210        220        230        240 
SNDTFPTAIH ISVGMELNER LVPAVTHLRD ALKSKSDEFK DIIKIGRTHL MDAVPLTLGQ 

       250        260        270        280        290        300 
EFSGYTQQLT NGLERIKGCL PRVYELALGG TAVGTGLNTR KGFAEKVAKR ISELTCLPFV 

       310        320        330        340        350        360 
SAPNKFEALA ARDAMVEVHG VLNTIAVSLM KIANDIRLLG SGPRCGLGEL MLPENEPGSS 

       370        380        390        400        410        420 
IMPGKVNPTQ CESMTMLCAQ VMGNQVAVTI GGSNGHFELN VFKPLVVSNV LRSIRLLADG 

       430        440        450        460        470        480 
SMTFSKNCVE GLQANKERID KIMNESLMLV TALNPHIGYD KAALIAKTAH KNKTTLKEEA 

       490        500 
LKTGITEEQF KEWVNPKEML GPK 

References and documentation are available.

Number of amino acids: 503

Molecular weight: 54518.17

Theoretical pI: 9.00

Amino acid composition: 
Ala (A)  36	  7.2%
Arg (R)  19	  3.8%
Asn (N)  31	  6.2%
Asp (D)  21	  4.2%
Cys (C)  11	  2.2%
Gln (Q)  15	  3.0%
Glu (E)  31	  6.2%
Gly (G)  49	  9.7%
His (H)  11	  2.2%
Ile (I)  26	  5.2%
Leu (L)  50	  9.9%
Lys (K)  44	  8.7%
Met (M)  21	  4.2%
Phe (F)  14	  2.8%
Pro (P)  22	  4.4%
Ser (S)  29	  5.8%
Thr (T)  29	  5.8%
Trp (W)   3	  0.6%
Tyr (Y)   6	  1.2%
Val (V)  35	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2383
Hydrogen    H	      3897
Nitrogen    N	       675
Oxygen      O	       718
Sulfur      S	        32

Formula: C2383H3897N675O718S32
Total number of atoms: 7705

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26065
Abs 0.1% (=1 g/l)   0.478, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   0.467, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.33
This classifies the protein as stable.



Aliphatic index: 86.26

Grand average of hydropathicity (GRAVY): -0.236