ProtParam
User-provided sequence:
10 20 30 40 50 60
MLKSASQNFF RAYSSRIGRY AATASGKLAQ SRLSNIPTPK ALKKFLDEYI VGQEIGKKVL
70 80 90 100 110 120
SVAVYNHYLR INDKQKKGEL QRQRELMERE KIADDRDEPI FSGNSESKAG WRNLQRQFNL
130 140 150 160 170 180
AGREVDEDLE LSKSNVLVVG PSGSGKTLLA TTLAKILNVP IAITDCTQLT QAGYIGEDVE
190 200 210 220 230 240
VCIERLLVNA EFDVARAEKG IIVLDEIDKL AKPAASIGTK DVSGEGVQQS LLKIIEGHKV
250 260 270 280 290 300
EITVKRPVKH DIDGQKNQTT TKKDEVFVVD TSNILFMIMG AFVGLDKHIV KRIEDMKKIQ
310 320 330 340 350 360
KAGESVESSN SKEVEKERAK KFRFSNTLEQ VELDNGKKVC ALDLTTPTDL VSFGLIPELI
370 380 390 400 410 420
GRVPIITALQ PLQRDDLFHI LKEPKNALLD QYEYIFKQFG VRLCVTQKAL KKVAQFALKE
430 440 450 460 470 480
GTGARGLRGI MERLLLNVNY DCPGSNIAYV LIDEATVDSL QETEHSLASQ VDVKYYSGDE
490 500 510 520
KDSLIRDVSE EDKKLGVMLE KELGHSANIH TPTIPKRSLT
References and
documentation are available.
Number of amino acids: 520
Molecular weight: 57945.54
Theoretical pI: 7.59
Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 2565
Hydrogen H 4197
Nitrogen N 709
Oxygen O 787
Sulfur S 12
Formula: C
2565H
4197N
709O
787S
12
Total number of atoms: 8270
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23630
Abs 0.1% (=1 g/l) 0.408, assuming all pairs of Cys residues form cystines
Ext. coefficient 23380
Abs 0.1% (=1 g/l) 0.403, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.03
This classifies the protein as stable.
Aliphatic index: 98.04
Grand average of hydropathicity (GRAVY): -0.339