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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKSIFAKRF ASTGSYPGST RITLPRRPAK KIQLGKSRPA IYHQFNVKME LSDGSVVIRR

70 80 90 100 110 120
SQYPKGEIRL IQDQRNNPLW NPSRDDLVVV DANSGGSLDR FNKRYSSLFS VDSTTPNSSS

130 140 150 160 170
ETVELSEENK KKTQIKKEEK EDVSEKAFGM DDYLSLLDDS EQQIKSGKLA SKKRDKK


References and documentation are available.

Number of amino acids: 177 Molecular weight: 20090.70 Theoretical pI: 9.72
Amino acid composition: 
Ala (A) 7 4.0% Arg (R) 13 7.3% Asn (N) 8 4.5% Asp (D) 13 7.3% Cys (C) 0 0.0% Gln (Q) 8 4.5% Glu (E) 11 6.2% Gly (G) 9 5.1% His (H) 1 0.6% Ile (I) 9 5.1% Leu (L) 14 7.9% Lys (K) 21 11.9% Met (M) 3 1.7% Phe (F) 6 3.4% Pro (P) 8 4.5% Ser (S) 24 13.6% Thr (T) 7 4.0% Trp (W) 1 0.6% Tyr (Y) 5 2.8% Val (V) 9 5.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 24 Total number of positively charged residues (Arg + Lys): 34 Atomic composition: Carbon C 876 Hydrogen H 1428 Nitrogen N 256 Oxygen O 278 Sulfur S 3 Formula: C876H1428N256O278S3 Total number of atoms: 2841 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 12950 Abs 0.1% (=1 g/l) 0.645 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 53.21 This classifies the protein as unstable. Aliphatic index: 69.38 Grand average of hydropathicity (GRAVY): -0.932