ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKTTESKEN EITKEQARWM YEKMLEIRKF EDKVHELFAQ GVLPGFVHLY AGEEAVAVGV 

        70         80         90        100        110        120 
CAHLTDSDSI TSTHRGHGHC IAKGCDLNGM MAELFGKATG LCKGKGGSMH IADLDKGMLG 

       130        140        150        160        170        180 
ANGIVGGGFP LACGSALTAK YKGTKDVSVC FFGDGANNEG TFHEGVNLAA IWKLPVIFIA 

       190        200        210        220        230        240 
ENNGYGEATT FEYASSCDSI ADRAKAYNIP GVQVDGKDLL AVYKAAEEAV ERARNGDGPT 

       250        260        270        280        290        300 
IIECMTYRNY GHFEGEAQTY KTSEEKEEHL NEKDAIVNFR KHLIHEALLT ESELVDMEKA 

       310        320        330 
VDEAVQKSIE FSENSPYPED EELLKDVYVS YK 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 36373.89

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  33	  9.9%
Arg (R)   8	  2.4%
Asn (N)  14	  4.2%
Asp (D)  18	  5.4%
Cys (C)   8	  2.4%
Gln (Q)   5	  1.5%
Glu (E)  37	 11.1%
Gly (G)  33	  9.9%
His (H)  12	  3.6%
Ile (I)  16	  4.8%
Leu (L)  24	  7.2%
Lys (K)  26	  7.8%
Met (M)   9	  2.7%
Phe (F)  13	  3.9%
Pro (P)   7	  2.1%
Ser (S)  16	  4.8%
Thr (T)  17	  5.1%
Trp (W)   2	  0.6%
Tyr (Y)  13	  3.9%
Val (V)  21	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1600
Hydrogen    H	      2483
Nitrogen    N	       427
Oxygen      O	       508
Sulfur      S	        17

Formula: C1600H2483N427O508S17
Total number of atoms: 5035

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30870
Abs 0.1% (=1 g/l)   0.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.835, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.41
This classifies the protein as stable.



Aliphatic index: 75.27

Grand average of hydropathicity (GRAVY): -0.356