ProtParam
User-provided sequence:
10 20 30 40 50 60
MLKTTESKEN EITKEQARWM YEKMLEIRKF EDKVHELFAQ GVLPGFVHLY AGEEAVAVGV
70 80 90 100 110 120
CAHLTDSDSI TSTHRGHGHC IAKGCDLNGM MAELFGKATG LCKGKGGSMH IADLDKGMLG
130 140 150 160 170 180
ANGIVGGGFP LACGSALTAK YKGTKDVSVC FFGDGANNEG TFHEGVNLAA IWKLPVIFIA
190 200 210 220 230 240
ENNGYGEATT FEYASSCDSI ADRAKAYNIP GVQVDGKDLL AVYKAAEEAV ERARNGDGPT
250 260 270 280 290 300
IIECMTYRNY GHFEGEAQTY KTSEEKEEHL NEKDAIVNFR KHLIHEALLT ESELVDMEKA
310 320 330
VDEAVQKSIE FSENSPYPED EELLKDVYVS YK
References and
documentation are available.
Number of amino acids: 332
Molecular weight: 36373.89
Theoretical pI: 4.99
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1600
Hydrogen H 2483
Nitrogen N 427
Oxygen O 508
Sulfur S 17
Formula: C
1600H
2483N
427O
508S
17
Total number of atoms: 5035
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30870
Abs 0.1% (=1 g/l) 0.849, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.835, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.41
This classifies the protein as stable.
Aliphatic index: 75.27
Grand average of hydropathicity (GRAVY): -0.356