ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLKVEKFKKL KRFEVYYCLK NSFLEEVDIE MKYSCSITTI MSNGSASLLM NWEELTPGHC 

        70         80         90        100 
FTSYTTNPIA GDYGLNASAI DGHTEELVAT HPAGTLENAT Q 

References and documentation are available.

Number of amino acids: 101

Molecular weight: 11289.82

Theoretical pI: 5.05

Amino acid composition: 
Ala (A)   7	  6.9%
Arg (R)   1	  1.0%
Asn (N)   6	  5.9%
Asp (D)   3	  3.0%
Cys (C)   3	  3.0%
Gln (Q)   1	  1.0%
Glu (E)  10	  9.9%
Gly (G)   6	  5.9%
His (H)   3	  3.0%
Ile (I)   5	  5.0%
Leu (L)  10	  9.9%
Lys (K)   7	  6.9%
Met (M)   4	  4.0%
Phe (F)   4	  4.0%
Pro (P)   3	  3.0%
Ser (S)   8	  7.9%
Thr (T)  10	  9.9%
Trp (W)   1	  1.0%
Tyr (Y)   5	  5.0%
Val (V)   4	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 8

Atomic composition:

Carbon      C	       500
Hydrogen    H	       775
Nitrogen    N	       125
Oxygen      O	       158
Sulfur      S	         7

Formula: C500H775N125O158S7
Total number of atoms: 1565

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   1.158, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   1.147, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 25.17
This classifies the protein as stable.



Aliphatic index: 76.34

Grand average of hydropathicity (GRAVY): -0.230