ProtParam
User-provided sequence:
10 20 30 40 50 60
MLKVPLSDVL SQKMLFLKSF RYFHCTKYFS RDNASSTTDI FRNAMKRKRE LANLKEQSHG
70 80 90 100 110 120
NVARNAAFPK EYIKRPKQVP RNATNRKKIL ITWSTGTDRA KEAANSVVSE IFKKNHKGNI
130 140 150 160 170 180
KVVDPTTHRI EASNIRYFAK GIDLDKVGLS IVNVEQIDNE NQIPLVKIVE SRVALKKYSD
190 200 210 220 230 240
FLAKKKEKEL MELGVLNKSY KNLVTDKKED NLKHIKISWQ IESDDLKRQK AHEIVSLLKK
250 260 270 280 290 300
GNKVTLYLDD KNNINSNNWL ENFEELDRSQ KGEPPRLPES VFQKRAAVLE TLKEIVSEYA
310 320 330 340 350
NDPVLLGNMN SKMIMKLIPK DVKPQNNDKR ALKELRKKER QEKLQKRIQR KKMNEM
References and
documentation are available.
Number of amino acids: 356
Molecular weight: 41461.09
Theoretical pI: 9.90
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 1837
Hydrogen H 3027
Nitrogen N 533
Oxygen O 535
Sulfur S 10
Formula: C
1837H
3027N
533O
535S
10
Total number of atoms: 5942
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.685, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.685, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.34
This classifies the protein as stable.
Aliphatic index: 85.42
Grand average of hydropathicity (GRAVY): -0.814