ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLDVNTNHT LMHDAHVHEH CLIKSIRDDG ALHSWSDSSK VFYPKSFYAT ATNKKNNKLA 

        70         80         90        100        110        120 
SASMNKTATS NRTVSDEIYF HSTKPQFDGQ GSAERTRTLT KRNSFKRTRI LKARDDSELL 

       130        140        150        160        170        180 
NENRSSLMTP SLSSVMSQVR KTNSAKTLSG ECPIHEGHLT QSIKRKFSEE AQSDCSSLSS 

       190        200        210        220        230        240 
SKLHPLTDDI ADAVDLQTPA IGDEVLAEPV VPKMKIININ DLDLFDDWEV KDLVDIFPPV 

       250        260        270        280        290        300 
YERRPRSSSA LSLVSASSDA KLRPTSVDFQ IIDKKGGKTS RRKSRSKSTT ENMIYENDLV 

       310        320        330        340        350        360 
ELEQWPSASP SPETDGSIAS SELLPNKRIR QKSLNTNFLK LYSIETSCKR KSILPEVEVD 

       370        380        390        400        410        420 
DHLLKQLTYS EIRSLEIKKE PNVSTNDIKL ALITRKKLWS DMVHETRNDL FGDSTPWNLH 

       430        440        450        460        470        480 
FVATTSNTEP SQGRESASEH ATADLKSSLV RVHSDVKPWF NNGGTMLKPC GKLNLGKVTN 

       490        500 
KTSAPTREIQ YVVKGWCDSR FL 

References and documentation are available.

Number of amino acids: 502

Molecular weight: 56356.52

Theoretical pI: 8.77

Amino acid composition: 
Ala (A)  26	  5.2%
Arg (R)  28	  5.6%
Asn (N)  27	  5.4%
Asp (D)  35	  7.0%
Cys (C)   6	  1.2%
Gln (Q)  12	  2.4%
Glu (E)  30	  6.0%
Gly (G)  16	  3.2%
His (H)  15	  3.0%
Ile (I)  24	  4.8%
Leu (L)  48	  9.6%
Lys (K)  43	  8.6%
Met (M)   9	  1.8%
Phe (F)  14	  2.8%
Pro (P)  23	  4.6%
Ser (S)  66	 13.1%
Thr (T)  38	  7.6%
Trp (W)   7	  1.4%
Tyr (Y)   8	  1.6%
Val (V)  27	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2454
Hydrogen    H	      3951
Nitrogen    N	       705
Oxygen      O	       784
Sulfur      S	        15

Formula: C2454H3951N705O784S15
Total number of atoms: 7909

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50795
Abs 0.1% (=1 g/l)   0.901, assuming all pairs of Cys residues form cystines


Ext. coefficient    50420
Abs 0.1% (=1 g/l)   0.895, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.81
This classifies the protein as unstable.



Aliphatic index: 76.71

Grand average of hydropathicity (GRAVY): -0.644