ProtParam
User-provided sequence:
10 20 30 40 50 60
MLLELISYAG TVSGFLFLTL SIASGLYYIS ELVEEHTEPT RRFLTRAIYG IILILILLLL
70 80 90 100 110 120
LDGFPFKLTL FSIACYIVYY QNLKSFPFIS LTSPTFLLSC VCVVLNHYFW FKYFNDTEVP
130 140 150 160 170 180
PQFKFDPNYI PRRRASFAEV ASFFGICVWF IPFALFVSLS AGDYVLPTTS EQHMAKKNDD
190 200 210 220
ITTNNQPKFR KRAVGLARVV INSVRKYIYS LARVFGYEIE PDFDRLAV
References and
documentation are available.
Number of amino acids: 228
Molecular weight: 26283.78
Theoretical pI: 8.60
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 1245
Hydrogen H 1883
Nitrogen N 293
Oxygen O 321
Sulfur S 6
Formula: C
1245H
1883N
293O
321S
6
Total number of atoms: 3748
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32110
Abs 0.1% (=1 g/l) 1.222, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 1.212, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.32
This classifies the protein as unstable.
Aliphatic index: 108.16
Grand average of hydropathicity (GRAVY): 0.425