ProtParam
User-provided sequence:
10 20 30 40 50 60
MLLFEQPVDL EKNNEDDTNI KPFAISRHFL LKLLLCGIIL IELLLYSKCP KPIDNGPRTI
70 80 90 100 110 120
ANRSNTYFNG THDFKTLTIL ISIDGFHPRL IDAKYTPFLY NLHNLRSPYD MNITTAPYMI
130 140 150 160 170 180
PSFPTQTFPN HWSMVTGKYP IEHGIVSNIF WDNFTSSEFR PNNLDARIWS NTADPIWQLL
190 200 210 220 230 240
QTESQGEYKV ATHMWPGSEV VYEDHGDVPR ERMPFYFGKF NQWEKLQDKL AQIFRYIDMP
250 260 270 280 290 300
QLKDRPELVI SYIPNVDSYG HSFGYDLRDK RLQKLIGEVD GFFLDLIEGL QKRNLLKISN
310 320 330 340 350 360
VMIVSDHGMS NVNANDGEHV VVWERVFPAD AMSAFISHLY NEGPMMMVCL KNPRDKQWIC
370 380 390 400 410 420
DLIEAQLEKA YGDEISRKFH VILKEDFDPS WKYFQYDNRK HRYDDRVGDI WILADEYYAI
430 440 450 460 470 480
VKEMGDVPIG IMGTHGYNFN NCSDMASIFI GMGPMFNNEV VPPFENIEVY NMLIKASALL
490
GEEKTKKEKS LLQ
References and
documentation are available.
Number of amino acids: 493
Molecular weight: 57354.61
Theoretical pI: 5.43
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2608
Hydrogen H 3959
Nitrogen N 671
Oxygen O 742
Sulfur S 24
Formula: C
2608H
3959N
671O
742S
24
Total number of atoms: 8004
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 89520
Abs 0.1% (=1 g/l) 1.561, assuming all pairs of Cys residues form cystines
Ext. coefficient 89270
Abs 0.1% (=1 g/l) 1.556, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.56
This classifies the protein as stable.
Aliphatic index: 85.01
Grand average of hydropathicity (GRAVY): -0.347