ProtParam
User-provided sequence:
10 20 30 40 50 60
MLLFRVLENL RACTEVSARN RHWTSIVGLS VRLSSTKAAP RYRRMDYQDA VCTLNTLQTN
70 80 90 100 110 120
ASALEQVKRE RGHPQLQLQA MRGFLQRAGL KVEELDRLNI IHVTGTKGKG STCAFTEKIL
130 140 150 160 170 180
RNYGFRTGFY SSPHLVQVRE RIRINGQPIG KELFTKYFWQ VYGRLEETKD AHGGSMPAYF
190 200 210 220 230 240
RFLTILAFHV FLQEKVDLAV IEVGIGGAYD CTNIIRRPWG CGISSLGIDH TSILGDTIEK
250 260 270 280 290 300
IAWQKGGIFK PGVPAFTVKQ PDGPMKVLQE RAEEVGCSLS VCPDLDEYES EGSLSLGLAG
310 320 330 340 350 360
QHQRSNASLA LQLSHTWLQR HVLTDPASPV EFSGVSSAPA FQPSPAMIKG LAETEWPGRN
370 380 390 400 410 420
QTLKHGPVTY YMDGAHTTRS MQACVRWFNE VATQHERNAG GSVLRVLLFN ATGERDCAAM
430 440 450 460 470 480
LKLLAPCHFD FAVFCPNITE AIASCNADQQ NFNVSVENML TRCLDNQQSW RVLNGLEEKP
490 500 510 520 530 540
EAELLIGGGL PLVAERHGDT LVFPCILSAL QWISQGRDSV LADANKHTIP VKPSINAKAA
550 560 570
SLREAQNIHV LITGSLHLVG GALKHLDPAL NS
References and
documentation are available.
Number of amino acids: 572
Molecular weight: 63161.29
Theoretical pI: 8.32
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2798
Hydrogen H 4431
Nitrogen N 803
Oxygen O 817
Sulfur S 24
Formula: C
2798H
4431N
803O
817S
24
Total number of atoms: 8873
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66765
Abs 0.1% (=1 g/l) 1.057, assuming all pairs of Cys residues form cystines
Ext. coefficient 65890
Abs 0.1% (=1 g/l) 1.043, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.78
This classifies the protein as unstable.
Aliphatic index: 89.88
Grand average of hydropathicity (GRAVY): -0.169