ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLFSSFFSH NKCPKQKKGP QPLKVVKGTS KSCAKQGKIK GKEKLWQAKT GRLVMSETGE 

        70         80         90        100        110        120 
TSEYYKQALE EYKEVQEDED PDVWDTRISK TGCYVENLAL QLCHAETGDW RQCFNEMALF 

       130        140        150 
RKCWEKNGNR ERVSTVDVDG TTSKDSEKKK 

References and documentation are available.

Number of amino acids: 150

Molecular weight: 17252.58

Theoretical pI: 8.82

Amino acid composition: 
Ala (A)   6	  4.0%
Arg (R)   6	  4.0%
Asn (N)   5	  3.3%
Asp (D)   8	  5.3%
Cys (C)   6	  4.0%
Gln (Q)   8	  5.3%
Glu (E)  15	 10.0%
Gly (G)  10	  6.7%
His (H)   2	  1.3%
Ile (I)   2	  1.3%
Leu (L)  10	  6.7%
Lys (K)  22	 14.7%
Met (M)   3	  2.0%
Phe (F)   5	  3.3%
Pro (P)   4	  2.7%
Ser (S)  11	  7.3%
Thr (T)  10	  6.7%
Trp (W)   4	  2.7%
Tyr (Y)   4	  2.7%
Val (V)   9	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	       753
Hydrogen    H	      1195
Nitrogen    N	       211
Oxygen      O	       235
Sulfur      S	         9

Formula: C753H1195N211O235S9
Total number of atoms: 2403

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28335
Abs 0.1% (=1 g/l)   1.642, assuming all pairs of Cys residues form cystines


Ext. coefficient    27960
Abs 0.1% (=1 g/l)   1.621, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.33
This classifies the protein as stable.



Aliphatic index: 52.60

Grand average of hydropathicity (GRAVY): -0.999