ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLIRRTINA FLGCIHCNLT ATCILIAFVI TMYVVLVSEP ASVDGTMGNF LPFSKMDLAT 

        70         80         90        100        110        120 
KRDRPFYSNC VNTQDYLLNP SYIKQNASFV MLTRNGELED VIKTINSIEE HFNQWFHYPY 

       130        140        150        160        170        180 
VFLNDQPFEE DFKAKVRDVT VGALVEFGTI DEISWNFPSD VKDTFEFYNA IEDQGDRSIL 

       190        200        210        220        230        240 
YGNLESYHKM CRFYSGLFYK HPLVQKYEWY WRLEPDVEFF CDITYDPFLE MLRTNKKYGF 

       250        260        270        280        290        300 
TIIIPELYWT VPNLFRHTKS FISQKGVTLG SLWKLFTKDY DIFESDDPEL RDWINYDFQA 

       310        320        330        340        350        360 
KAKISEKIAI EQLLKKGDDF QQINDDKEGI MNLIHKARSR KHIVEDKFFN EEYNLCHFWS 

       370        380        390        400        410        420 
NFEIARLSVF DNDIYNSFFQ YLEKSGGFWK ERWGDAPVHS IGLSLTLDLD DVHYFRDIGY 

       430        440        450        460        470        480 
RHSTIQHCPH NAMGNEEFSY LASDSKFKRK NAAYDEGREF GCGCRCRCPK KKREIEDSMG 

       490        500        510        520 
FCVNIWVNLL NQQRGHERHV EALNGNEMEE HIREDYLRQF GN 

References and documentation are available.

Number of amino acids: 522

Molecular weight: 61727.88

Theoretical pI: 5.46

Amino acid composition: 
Ala (A)  20	  3.8%
Arg (R)  27	  5.2%
Asn (N)  33	  6.3%
Asp (D)  38	  7.3%
Cys (C)  13	  2.5%
Gln (Q)  16	  3.1%
Glu (E)  40	  7.7%
Gly (G)  27	  5.2%
His (H)  17	  3.3%
Ile (I)  36	  6.9%
Leu (L)  42	  8.0%
Lys (K)  33	  6.3%
Met (M)  11	  2.1%
Phe (F)  41	  7.9%
Pro (P)  16	  3.1%
Ser (S)  29	  5.6%
Thr (T)  21	  4.0%
Trp (W)  11	  2.1%
Tyr (Y)  26	  5.0%
Val (V)  25	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2802
Hydrogen    H	      4182
Nitrogen    N	       730
Oxygen      O	       804
Sulfur      S	        24

Formula: C2802H4182N730O804S24
Total number of atoms: 8542

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    99990
Abs 0.1% (=1 g/l)   1.620, assuming all pairs of Cys residues form cystines


Ext. coefficient    99240
Abs 0.1% (=1 g/l)   1.608, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.14
This classifies the protein as unstable.



Aliphatic index: 76.00

Grand average of hydropathicity (GRAVY): -0.453