ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLKFEASSE LRLVDPSVSL TVLRKIRLSH LPDMTMTSES SGKKCGEGDG KVAGKSQRKV 

        70         80         90        100        110        120 
GLVYDETMCK HDTPNGKVDV ECPDRIRVIW EKLQLAGVTQ RCVVLGGSKA EDKHLKLVHT 

       130        140        150        160        170        180 
KKHVNLVKSI STKKKDSRRN KIASQLDSIY LNGGSSEAAY LAAGSVVKVA EKVAEGELDC 

       190        200        210        220        230        240 
GFAIVRPPGH HAESDEAMGF CLFNNVAVAA SFLLNERPDL DVKKILIVDW DIHHGNGTQK 

       250        260        270        280        290        300 
MFWKDSRVLI FSVHRHDHGS FYPFGDDGDF NMVGEGPGEG FNINVPWEQG GCGDADYLAV 

       310        320        330        340        350        360 
WNHILIPVTK EFKPDIILLS AGFDAAIGDP LGGCCVTPYG YSVMLKKLME FAHGKIVLAL 

       370        380        390        400        410        420 
EGGYNLESLG KSSLACVQVL LEDKQIHGSS ETYPLESTRR VIQAVRERLC TYWPSLDASM 

       430        440        450        460        470        480 
ASNENLKNPS AERNSADALL REVEELKSLM AARDGELEAR RKELKAKNKE LEANEKELEA 

       490        500        510        520        530        540 
GLMLIRARED VICGLHAKIE SLQQERDEAV AKAERIDKEL QEDRARSQEF KEDTEFCLST 

       550        560        570        580        590        600 
LRREKELAIM AKNKDLEAKE KELEARLMLV HAREDKIHAK IERLQQERDE AVAKAERIDK 

       610        620        630        640        650        660 
ELQEDRSRSR VGNGSFAFSQ EFYEDMDLDE LEPLSPEFNE DMDSEELEPF QVIKKNMERS 

       670        680        690        700        710        720 
HKKFIKDMEC IKFIAIMAMA GESSGKKIGD CDGKVAGNRQ RKVGLIYDET MCKHDTPDGE 

       730        740        750        760        770        780 
DHPECPDRIR VIWEKLQLAG VSQRCVVLGS SKAEDKHLQL VHTKDHVNLV KSISTKQKDY 

       790        800        810        820        830        840 
RRNRIASQLN SIYLNGGSSE AAYLAAGSVV KLAEKVAEGE LDCGFAIVRP PGHHAEADEA 

       850        860        870        880        890        900 
MGFCLFNNVA VAASFLLNER PDLGVKKILI VDWDVHHGNG TQKMFWKDPR VLFFSVHRHE 

       910        920        930        940        950        960 
YGGFYPAGDD GDYNMVGEGP GEGFNINVPW DQGRCGDADY LAAWDHILIP VAREFNPDVI 

       970        980        990       1000       1010       1020 
FLSAGFDAAI NDPLGGCCVT PYGYSVMLKK LMEFAQGKIV LALEGGYNLD SIAKSSLACV 

      1030       1040       1050       1060       1070       1080 
QVLLEDKQIQ GPPEAYPFES TWRVIQAVWL YIILSFNFLI SQSETTFHKW AVVLMLLVDQ 

      1090       1100 
NSSVSCGFRC ARGYAHIGLH LQMNYRGS 

References and documentation are available.

Number of amino acids: 1108

Molecular weight: 123609.88

Theoretical pI: 5.64

Amino acid composition: 
Ala (A)  90	  8.1%
Arg (R)  57	  5.1%
Asn (N)  41	  3.7%
Asp (D)  75	  6.8%
Cys (C)  26	  2.3%
Gln (Q)  34	  3.1%
Glu (E)  96	  8.7%
Gly (G)  85	  7.7%
His (H)  34	  3.1%
Ile (I)  56	  5.1%
Leu (L) 107	  9.7%
Lys (K)  83	  7.5%
Met (M)  28	  2.5%
Phe (F)  41	  3.7%
Pro (P)  37	  3.3%
Ser (S)  74	  6.7%
Thr (T)  25	  2.3%
Trp (W)  14	  1.3%
Tyr (Y)  26	  2.3%
Val (V)  79	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 171
Total number of positively charged residues (Arg + Lys): 140

Atomic composition:

Carbon      C	      5453
Hydrogen    H	      8623
Nitrogen    N	      1519
Oxygen      O	      1651
Sulfur      S	        54

Formula: C5453H8623N1519O1651S54
Total number of atoms: 17300

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117365
Abs 0.1% (=1 g/l)   0.949, assuming all pairs of Cys residues form cystines


Ext. coefficient   115740
Abs 0.1% (=1 g/l)   0.936, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.21
This classifies the protein as stable.



Aliphatic index: 86.17

Grand average of hydropathicity (GRAVY): -0.344