ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK NEDNNEDAKL 

        70         80         90        100        110        120 
NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI DESIRRLEDA VLKQESNRIQ 

       130        140        150        160        170        180 
EERKEKEEEN GPSKAKSNRT KEQGYFEGNN SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL 

       190        200        210        220        230        240 
FQIGLTFFLL SFLLDLLNSL EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG 

       250        260        270        280        290        300 
KNQADNYGRN FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 

       310        320        330        340        350        360 
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV AGCDEAKEEI 

       370        380        390        400        410        420 
MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA TAGEAGVPFY FVSGSEFVEM 

       430        440        450        460        470        480 
FVGVGAARVR DLFKTARENA PSIVFIDEID AIGKARQKGN FSGANDEREN TLNQMLVEMD 

       490        500        510        520        530        540 
GFTPADHVVV LAGTNRPDIL DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE 

       550        560        570        580        590        600 
IFDLKNRLAA LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 

       610        620        630        640        650        660 
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP GDIFLLTEQQ 

       670        680        690        700        710        720 
LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM VTELGMSDKI GWVNYQKRDD 

       730        740        750        760        770        780 
SDLTKPFSDE TGDIIDSEVY RIVQECHDRC TKLLKEKAED VEKIAQVLLK KEVLTREDMI 

       790        800        810        820 
DLLGKRPFPE RNDAFDKYLN DYETEKIRKE EEKNEKRNEP KPSTN 

References and documentation are available.

Number of amino acids: 825

Molecular weight: 93276.30

Theoretical pI: 7.22

Amino acid composition: 
Ala (A)  60	  7.3%
Arg (R)  49	  5.9%
Asn (N)  46	  5.6%
Asp (D)  51	  6.2%
Cys (C)   5	  0.6%
Gln (Q)  27	  3.3%
Glu (E)  74	  9.0%
Gly (G)  53	  6.4%
His (H)  13	  1.6%
Ile (I)  49	  5.9%
Leu (L)  74	  9.0%
Lys (K)  76	  9.2%
Met (M)  16	  1.9%
Phe (F)  36	  4.4%
Pro (P)  40	  4.8%
Ser (S)  45	  5.5%
Thr (T)  34	  4.1%
Trp (W)   7	  0.8%
Tyr (Y)  21	  2.5%
Val (V)  49	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 125
Total number of positively charged residues (Arg + Lys): 125

Atomic composition:

Carbon      C	      4146
Hydrogen    H	      6606
Nitrogen    N	      1154
Oxygen      O	      1249
Sulfur      S	        21

Formula: C4146H6606N1154O1249S21
Total number of atoms: 13176

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70040
Abs 0.1% (=1 g/l)   0.751, assuming all pairs of Cys residues form cystines


Ext. coefficient    69790
Abs 0.1% (=1 g/l)   0.748, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.19
This classifies the protein as unstable.



Aliphatic index: 82.64

Grand average of hydropathicity (GRAVY): -0.571