ProtParam
User-provided sequence:
10 20 30 40 50 60
MLLPILLHFL LLITQLNGSP AEVRLINDLM SGYVREERPT LDSSKPVVVS LGVFLQQIIN
70 80 90 100 110 120
LSEKEEQLEV NAWLKFQWRD ENLRWEPTAY ENVTDLRHPP DALWTPDILL YNSVDSEFDS
130 140 150 160 170 180
SYKVNLVNYH TGNINWMPPG IFKVSCKLDI YWFPFDEQVC YFKFGSWTYT RDKIQLEKGD
190 200 210 220 230 240
FDFSEFIPNG EWIIIDYRTN ITVKQYECCP EQYEDITFTL HLRRRTLYYS FNLIAPVLLT
250 260 270 280 290 300
MILVILGFTV SPETCEKVGL QISVSLAICI FLTIMSELTP QTSEAVPLLG VFFHTCNFIS
310 320 330 340 350 360
VLATSFTVYV QSFHFRNQHV HERMDFWMRF ILLEWSPWLL RMKMPDRENN FQTLTESWKG
370 380 390 400 410 420
RNRRESMART AFEYADGPVT QIHSMGIMLK DNFEELIYQV KQEKIADEKG IERLRVLQKI
430 440 450 460 470
YDHVKMIREH DDDNDEDSRV ALEWRFAAIV VDRLCLLAFS LLIVVVSIII ALRAPYLFA
References and
documentation are available.
Number of amino acids: 479
Molecular weight: 56334.06
Theoretical pI: 5.18
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2584
Hydrogen H 3959
Nitrogen N 653
Oxygen O 718
Sulfur S 21
Formula: C
2584H
3959N
653O
718S
21
Total number of atoms: 7935
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 98820
Abs 0.1% (=1 g/l) 1.754, assuming all pairs of Cys residues form cystines
Ext. coefficient 98320
Abs 0.1% (=1 g/l) 1.745, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.67
This classifies the protein as unstable.
Aliphatic index: 100.88
Grand average of hydropathicity (GRAVY): -0.031