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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLPILLHFL LLITQLNGSP AEVRLINDLM SGYVREERPT LDSSKPVVVS LGVFLQQIIN

70 80 90 100 110 120
LSEKEEQLEV NAWLKFQWRD ENLRWEPTAY ENVTDLRHPP DALWTPDILL YNSVDSEFDS

130 140 150 160 170 180
SYKVNLVNYH TGNINWMPPG IFKVSCKLDI YWFPFDEQVC YFKFGSWTYT RDKIQLEKGD

190 200 210 220 230 240
FDFSEFIPNG EWIIIDYRTN ITVKQYECCP EQYEDITFTL HLRRRTLYYS FNLIAPVLLT

250 260 270 280 290 300
MILVILGFTV SPETCEKVGL QISVSLAICI FLTIMSELTP QTSEAVPLLG VFFHTCNFIS

310 320 330 340 350 360
VLATSFTVYV QSFHFRNQHV HERMDFWMRF ILLEWSPWLL RMKMPDRENN FQTLTESWKG

370 380 390 400 410 420
RNRRESMART AFEYADGPVT QIHSMGIMLK DNFEELIYQV KQEKIADEKG IERLRVLQKI

430 440 450 460 470
YDHVKMIREH DDDNDEDSRV ALEWRFAAIV VDRLCLLAFS LLIVVVSIII ALRAPYLFA


References and documentation are available.

Number of amino acids: 479 Molecular weight: 56334.06 Theoretical pI: 5.18
Amino acid composition: 
Ala (A) 19 4.0% Arg (R) 27 5.6% Asn (N) 21 4.4% Asp (D) 27 5.6% Cys (C) 8 1.7% Gln (Q) 18 3.8% Glu (E) 36 7.5% Gly (G) 15 3.1% His (H) 11 2.3% Ile (I) 37 7.7% Leu (L) 56 11.7% Lys (K) 19 4.0% Met (M) 13 2.7% Phe (F) 30 6.3% Pro (P) 21 4.4% Ser (S) 29 6.1% Thr (T) 26 5.4% Trp (W) 13 2.7% Tyr (Y) 18 3.8% Val (V) 35 7.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 63 Total number of positively charged residues (Arg + Lys): 46 Atomic composition: Carbon C 2584 Hydrogen H 3959 Nitrogen N 653 Oxygen O 718 Sulfur S 21 Formula: C2584H3959N653O718S21 Total number of atoms: 7935 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 98820 Abs 0.1% (=1 g/l) 1.754, assuming all pairs of Cys residues form cystines Ext. coefficient 98320 Abs 0.1% (=1 g/l) 1.745, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 44.67 This classifies the protein as unstable. Aliphatic index: 100.88 Grand average of hydropathicity (GRAVY): -0.031