ProtParam
User-provided sequence:
10 20 30 40 50 60
MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS
70 80 90 100 110 120
NGSSEVYKPK EEVVKYILHG KFTKNNTHLT FSSVVEDKNF HKNKGLTYND TMLYYLNLPQ
130 140 150 160 170 180
KVKISLSTGC LGFRKAARGE YEAAFQTSGR MFELIKEKNM LNKDIEVVMD DFGKGRAAFI
190 200 210
SALVGKEGAS VVKKVVKISD ATKLKFGGVR SPKMRRL
References and
documentation are available.
Number of amino acids: 217
Molecular weight: 24563.35
Theoretical pI: 9.90
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1100
Hydrogen H 1753
Nitrogen N 305
Oxygen O 316
Sulfur S 8
Formula: C
1100H
1753N
305O
316S
8
Total number of atoms: 3482
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27055
Abs 0.1% (=1 g/l) 1.101, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 1.096, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.51
This classifies the protein as stable.
Aliphatic index: 74.93
Grand average of hydropathicity (GRAVY): -0.465