ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLLRSFRTVE LLRRISTSSV SGYRTSPSLL QRCNGFQSYL PHRVTTTESP FPSHISRFCS 

        70         80         90        100        110        120 
SQSANSQNEN RHTTLSSPVS SRRVNNRKIS RRRKAGKSLS ISPAADAVDL ALDSVVKIFT 

       130        140        150        160        170        180 
VSTSPSYFLP WQNKSQRESM GSGFVISGRK IITNAHVVAD HSFVLVRKHG SSIKHRAEVQ 

       190        200        210        220        230        240 
AVGHECDLAI LVVDSEVFWE GMNALELGDI PFLQEAVAVV GYPQGGDNIS VTKGVVSRVE 

       250        260        270        280        290        300 
PTQYVHGATQ LMAIQIDAAI NPGNSGGPAI MGNKVAGVAF QNLSGAENIG YIIPTPVIKH 

       310        320        330        340        350        360 
FINGVEECGK YIGFCSMGVS CQPMENGELR SGFQMSSEMT GVLVSKINPL SDAHKILKKD 

       370        380        390        400        410        420 
DVLLAFDGVP IANDGTVPFR NRERITFDHL VSMKKPDETA LVKVLREGKE HEFSITLRPL 

       430        440        450        460        470        480 
QPLVPVHQFD QLPSYYIFAG FVFVPLTQPY LHEYGEDWYN TSPRTLCHRA LKDLPKKAGQ 

       490        500        510        520        530        540 
QLVIVSQVLM DDINTGYERL AELQVNKVNG VEVNNLRHLC QLIENCNTEK LRIDLDDESR 

       550        560        570        580 
VIVLNYQSAK IATSLILKRH RIASAISSDL LIEQNLETEL ASCSAV 

References and documentation are available.

Number of amino acids: 586

Molecular weight: 64725.97

Theoretical pI: 8.23

Amino acid composition: 
Ala (A)  36	  6.1%
Arg (R)  34	  5.8%
Asn (N)  30	  5.1%
Asp (D)  25	  4.3%
Cys (C)  10	  1.7%
Gln (Q)  26	  4.4%
Glu (E)  33	  5.6%
Gly (G)  37	  6.3%
His (H)  18	  3.1%
Ile (I)  38	  6.5%
Leu (L)  54	  9.2%
Lys (K)  27	  4.6%
Met (M)  11	  1.9%
Phe (F)  22	  3.8%
Pro (P)  28	  4.8%
Ser (S)  59	 10.1%
Thr (T)  27	  4.6%
Trp (W)   3	  0.5%
Tyr (Y)  14	  2.4%
Val (V)  54	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2860
Hydrogen    H	      4576
Nitrogen    N	       810
Oxygen      O	       859
Sulfur      S	        21

Formula: C2860H4576N810O859S21
Total number of atoms: 9126

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37985
Abs 0.1% (=1 g/l)   0.587, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.577, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.46
This classifies the protein as unstable.



Aliphatic index: 94.10

Grand average of hydropathicity (GRAVY): -0.147